A quantitative determination of lipid bilayer deposition efficiency using AFM.
Milan, David C
Casford, Michael TL
Royal Society of Chemistry (RSC)
MetadataShow full item record
Wood, M. H., Milan, D. C., Nichols, R. J., Casford, M. T., & Horswell, S. L. (2021). A quantitative determination of lipid bilayer deposition efficiency using AFM.. RSC Adv, 11 (32), 19768-19778. https://doi.org/10.1039/d1ra01920a
The efficacy of a number of different methods for depositing a dimyristoylphosphatidylcholine (DMPC) lipid bilayer or DMPC-cholesterol (3 : 1) mixed bilayer onto a silicon substrate has been investigated in a quantitative manner using atomic force microscopy (AFM) image analysis to extract surface coverage. Complementary AFM-IR measurements were used to confirm the presence of the lipids. For the Langmuir-Blodgett/Schaefer deposition method at temperatures below the chain-melting transition temperature (T m), a large number of bilayer defects resulted when DMPC was deposited from a water subphase. Addition of calcium ions to the trough led to smaller, more frequent defects, whereas addition of cholesterol to the lipid mixture led to a vast improvement in bilayer coverage. Poor coverage was achieved for deposition at temperatures above T m. Formation of the deposited bilayer from vesicle fusion proved a more reliable method for all systems, with formation of near-complete bilayers within 60 seconds at temperatures above T m, although this method led to a higher probability of multilayer formation and rougher bilayer surfaces.
Leverhulme Trust (RPG-2016-158)
External DOI: https://doi.org/10.1039/d1ra01920a
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337724
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: firstname.lastname@example.org