A quantitative determination of lipid bilayer deposition efficiency using AFM.
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Publication Date
2021-05-27Journal Title
RSC Adv
ISSN
2046-2069
Publisher
Royal Society of Chemistry (RSC)
Volume
11
Issue
32
Pages
19768-19778
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Wood, M. H., Milan, D. C., Nichols, R. J., Casford, M. T., & Horswell, S. L. (2021). A quantitative determination of lipid bilayer deposition efficiency using AFM.. RSC Adv, 11 (32), 19768-19778. https://doi.org/10.1039/d1ra01920a
Abstract
The efficacy of a number of different methods for depositing a dimyristoylphosphatidylcholine (DMPC) lipid bilayer or DMPC-cholesterol (3 : 1) mixed bilayer onto a silicon substrate has been investigated in a quantitative manner using atomic force microscopy (AFM) image analysis to extract surface coverage. Complementary AFM-IR measurements were used to confirm the presence of the lipids. For the Langmuir-Blodgett/Schaefer deposition method at temperatures below the chain-melting transition temperature (T m), a large number of bilayer defects resulted when DMPC was deposited from a water subphase. Addition of calcium ions to the trough led to smaller, more frequent defects, whereas addition of cholesterol to the lipid mixture led to a vast improvement in bilayer coverage. Poor coverage was achieved for deposition at temperatures above T m. Formation of the deposited bilayer from vesicle fusion proved a more reliable method for all systems, with formation of near-complete bilayers within 60 seconds at temperatures above T m, although this method led to a higher probability of multilayer formation and rougher bilayer surfaces.
Sponsorship
Leverhulme Trust (RPG-2016-158)
Identifiers
35479201, PMC9033767
External DOI: https://doi.org/10.1039/d1ra01920a
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337724
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