Air pollution induces Staphylococcus aureus USA300 respiratory tract colonization mediated by specific bacterial genetic responses involving the global virulence gene regulators Agr and Sae.
Authors
Hussey, Shane JK
Corscadden, Louise
Purser, Lillie
Hall, Andie
Misra, Raju
Selley, Liza
Monks, Paul S
Ketley, Julian M
Andrew, Peter W
Publication Date
2022-09Journal Title
Environ Microbiol
ISSN
1462-2912
Publisher
Wiley
Language
en
Type
Article
This Version
AO
VoR
Metadata
Show full item recordCitation
Purves, J., Hussey, S. J., Corscadden, L., Purser, L., Hall, A., Misra, R., Selley, L., et al. (2022). Air pollution induces Staphylococcus aureus USA300 respiratory tract colonization mediated by specific bacterial genetic responses involving the global virulence gene regulators Agr and Sae.. Environ Microbiol https://doi.org/10.1111/1462-2920.16076
Description
Funder: MRC DTP IMPACT studentship
Funder: National Centre for Atmospheric Science Air Pollution Science Training Studentship Programme
Abstract
Exposure to particulate matter (PM), a major component of air pollution, is associated with exacerbation of chronic respiratory disease, and infectious diseases such as community-acquired pneumonia. Although PM can cause adverse health effects through direct damage to host cells, our previous study showed that PM can also impact bacterial behaviour by promoting in vivo colonization. In this study we describe the genetic mechanisms involved in the bacterial response to exposure to black carbon (BC), a constituent of PM found in most sources of air pollution. We show that Staphylococcus aureus strain USA300 LAC grown in BC prior to inoculation showed increased murine respiratory tract colonization and pulmonary invasion in vivo, as well as adhesion and invasion of human epithelial cells in vitro. Global transcriptional analysis showed that BC has a widespread effect on S. aureus transcriptional responses, altering the regulation of the major virulence gene regulators Sae and Agr and causing increased expression of genes encoding toxins, proteases and immune evasion factors. Together these data describe a previously unrecognized causative mechanism of air pollution-associated infection, in that exposure to BC can increase bacterial colonization and virulence factor expression by acting directly on the bacterium rather than via the host.
Keywords
Air Pollution, Animals, Bacterial Proteins, Carbon, Gene Expression Regulation, Bacterial, Humans, Mice, Particulate Matter, Peptide Hydrolases, Respiratory System, Staphylococcal Infections, Staphylococcus aureus, Virulence, Virulence Factors
Sponsorship
Leverhulme Trust (RPG‐2015‐183)
Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Program (HHSN272200700055C)
Identifiers
emi16076
External DOI: https://doi.org/10.1111/1462-2920.16076
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337760
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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