Show simple item record

dc.contributor.authorPurves, Jo
dc.contributor.authorHussey, Shane JK
dc.contributor.authorCorscadden, Louise
dc.contributor.authorPurser, Lillie
dc.contributor.authorHall, Andie
dc.contributor.authorMisra, Raju
dc.contributor.authorSelley, Liza
dc.contributor.authorMonks, Paul S
dc.contributor.authorKetley, Julian M
dc.contributor.authorAndrew, Peter W
dc.contributor.authorMorrissey, Julie A
dc.date.accessioned2022-06-07T08:11:37Z
dc.date.available2022-06-07T08:11:37Z
dc.date.issued2022-09
dc.date.submitted2022-02-03
dc.identifier.issn1462-2912
dc.identifier.otheremi16076
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337760
dc.descriptionFunder: MRC DTP IMPACT studentship
dc.descriptionFunder: National Centre for Atmospheric Science Air Pollution Science Training Studentship Programme
dc.description.abstractExposure to particulate matter (PM), a major component of air pollution, is associated with exacerbation of chronic respiratory disease, and infectious diseases such as community-acquired pneumonia. Although PM can cause adverse health effects through direct damage to host cells, our previous study showed that PM can also impact bacterial behaviour by promoting in vivo colonization. In this study we describe the genetic mechanisms involved in the bacterial response to exposure to black carbon (BC), a constituent of PM found in most sources of air pollution. We show that Staphylococcus aureus strain USA300 LAC grown in BC prior to inoculation showed increased murine respiratory tract colonization and pulmonary invasion in vivo, as well as adhesion and invasion of human epithelial cells in vitro. Global transcriptional analysis showed that BC has a widespread effect on S. aureus transcriptional responses, altering the regulation of the major virulence gene regulators Sae and Agr and causing increased expression of genes encoding toxins, proteases and immune evasion factors. Together these data describe a previously unrecognized causative mechanism of air pollution-associated infection, in that exposure to BC can increase bacterial colonization and virulence factor expression by acting directly on the bacterium rather than via the host.
dc.languageen
dc.publisherWiley
dc.subjectResearch article
dc.subjectResearch articles
dc.titleAir pollution induces Staphylococcus aureus USA300 respiratory tract colonization mediated by specific bacterial genetic responses involving the global virulence gene regulators Agr and Sae.
dc.typeArticle
dc.date.updated2022-06-07T08:11:37Z
prism.publicationNameEnviron Microbiol
dc.identifier.doi10.17863/CAM.85169
dcterms.dateAccepted2022-05-16
rioxxterms.versionofrecord10.1111/1462-2920.16076
rioxxterms.versionAO
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidPurves, Jo [0000-0002-4761-9949]
dc.contributor.orcidMorrissey, Julie A [0000-0001-9115-2218]
dc.identifier.eissn1462-2920
pubs.funder-project-idLeverhulme Trust (RPG‐2015‐183)
pubs.funder-project-idNetwork on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Program (HHSN272200700055C)
cam.issuedOnline2022-06


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record