Fluorescent Nanoparticles for Super-Resolution Imaging.
American Chemical Society (ACS)
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Li, W., Kaminski Schierle, G. S., Lei, B., Liu, Y., & Kaminski, C. F. (2022). Fluorescent Nanoparticles for Super-Resolution Imaging.. Chem Rev https://doi.org/10.1021/acs.chemrev.2c00050
Super-resolution imaging techniques that overcome the diffraction limit of light have gained wide popularity for visualizing cellular structures with nanometric resolution. Following the pace of hardware developments, the availability of new fluorescent probes with superior properties is becoming ever more important. In this context, fluorescent nanoparticles (NPs) have attracted increasing attention as bright and photostable probes that address many shortcomings of traditional fluorescent probes. The use of NPs for super-resolution imaging is a recent development and this provides the focus for the current review. We give an overview of different super-resolution methods and discuss their demands on the properties of fluorescent NPs. We then review in detail the features, strengths, and weaknesses of each NP class to support these applications and provide examples from their utilization in various biological systems. Moreover, we provide an outlook on the future of the field and opportunities in material science for the development of probes for multiplexed subcellular imaging with nanometric resolution.
CFK acknowledges funding from the UK Engineering and Physical Sciences Research Council, EPSRC (grants EP/L015889/1 and EP/H018301/1), the Wellcome Trust (grants 3-3249/Z/16/Z and 089703/Z/09/Z) and the UK Medical Research Council, MRC (grants MR/K015850/1 and MR/K02292X/1), MedImmune, and Infinitus (China) Ltd. WL acknowledges financial support from China Scholarship Council, the National Natural Science Foundations of China (Youth Science Foundation Project, Grant Nos. 52102042), Guangdong Basic and Applied Basic Research Foundation (2022A1515010452) and Macau Yong Scholars Program.
Engineering and Physical Sciences Research Council (EP/L015889/1)
Wellcome Trust (203249/Z/16/Z)
Engineering and Physical Sciences Research Council (EP/H018301/1)
Wellcome Trust (089703/Z/09/Z)
Medical Research Council (MR/K015850/1)
Medical Research Council (MR/K02292X/1)
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External DOI: https://doi.org/10.1021/acs.chemrev.2c00050
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337930
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