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dc.contributor.authorFalo-Sanjuan, Julia
dc.contributor.authorBray, Sarah
dc.date.accessioned2022-06-10T02:30:20Z
dc.date.available2022-06-10T02:30:20Z
dc.date.issued2022-05-18
dc.date.submitted2021-09-06
dc.identifier.issn2050-084X
dc.identifier.other73656
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/337980
dc.description.abstractCells sense and integrate external information from diverse sources that include mechanical cues. Shaping of tissues during development may thus require coordination between mechanical forces from morphogenesis and cell-cell signalling to confer appropriate changes in gene expression. By live-imaging Notch-induced transcription in real time, we have discovered that morphogenetic movements during Drosophila gastrulation bring about an increase in activity-levels of a Notch-responsive enhancer. Mutations that disrupt the timing of gastrulation resulted in concomitant delays in transcription up-regulation that correlated with the start of mesoderm invagination. As a similar gastrulation-induced effect was detected when transcription was elicited by the intracellular domain NICD, it cannot be attributed to forces exerted on Notch receptor activation. A Notch-independent vnd enhancer also exhibited a modest gastrulation-induced activity increase in the same stripe of cells. Together, these observations argue that gastrulation-associated forces act on the nucleus to modulate transcription levels. This regulation was uncoupled when the complex linking the nucleoskeleton and cytoskeleton (LINC) was disrupted, indicating a likely conduit. We propose that the coupling between tissue-level mechanics, arising from gastrulation, and enhancer activity represents a general mechanism for ensuring correct tissue specification during development and that Notch-dependent enhancers are highly sensitive to this regulation.
dc.languageen
dc.publishereLife Sciences Publications, Ltd
dc.subjectResearch Article
dc.subjectDevelopmental Biology
dc.subjectnotch
dc.subjectgastrulation
dc.subjecttranscription
dc.subjectlive-imaging
dc.subjecttissue-mechanics
dc.subjectD. melanogaster
dc.titleNotch-dependent and -independent transcription are modulated by tissue movements at gastrulation.
dc.typeArticle
dc.date.updated2022-06-10T02:30:18Z
prism.publicationNameElife
prism.volume11
dc.identifier.doi10.17863/CAM.85386
dcterms.dateAccepted2022-04-27
rioxxterms.versionofrecord10.7554/eLife.73656
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Devenport, Danelle
datacite.contributor.supervisorsenior_editor: Desplan, Claude
dc.contributor.orcidFalo-Sanjuan, Julia [0000-0002-3563-4789]
dc.contributor.orcidBray, Sarah [0000-0002-1642-599X]
dc.identifier.eissn2050-084X
pubs.funder-project-idMRC (MR/T014156/1)
pubs.funder-project-idWellcome Trust (212207/Z/18/Z)
pubs.funder-project-idWellcome Trust (102175/B/13/Z)
pubs.funder-project-idWellcome Trust (109144/Z/15/Z)
cam.issuedOnline2022-05-18


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