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dc.contributor.authorYan, Xingyu
dc.contributor.authorShang, Pengcheng
dc.contributor.authorYim-Im, Wannarat
dc.contributor.authorSun, Yankuo
dc.contributor.authorZhang, Jianqiang
dc.contributor.authorFirth, Andrew E
dc.contributor.authorLowe, James F
dc.contributor.authorFang, Ying
dc.date.accessioned2022-06-10T23:30:29Z
dc.date.available2022-06-10T23:30:29Z
dc.date.issued2022-08
dc.identifier.issn0042-6822
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338004
dc.description.abstractIn this study, we characterized an emerging porcine reproductive and respiratory syndrome virus (PRRSV) isolate UIL21-0712, which is a lineage 1C variant with ORF5 restriction fragment length polymorphism (RFLP) cutting pattern of 1-4-4. The UIL21-0712 genome sequence has 85.3% nucleotide identity with the prototypic PRRSV-2 strain VR2332. The nsp2 region is the most variable, and the -2/-1 programmed ribosome frameshifting (PRF) signal therein is distinct from historical PRRSV strains. Analysis of PRRSV sequences in GenBank revealed that the majority of the emerging PRRSV variants contain substitutions that disrupt the -1 PRF stop codon to generate a nsp2N protein with a C-terminal extension. Two of the -1 PRF stop codon variant patterns were identified to be predominantly circulating in the field. They demonstrated higher growth kinetics than the other variants, suggesting that the most dominant -1 PRF stop codon variant patterns may provide enhanced growth fitness for the virus.
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleMolecular characterization of emerging variants of PRRSV in the United States: new features of the -2/-1 programmed ribosomal frameshifting signal in the nsp2 region.
dc.typeArticle
dc.publisher.departmentDepartment of Pathology
dc.date.updated2022-06-06T11:11:48Z
prism.publicationDate2022
prism.publicationNameVirology
dc.identifier.doi10.17863/CAM.85409
dcterms.dateAccepted2022-06-01
rioxxterms.versionofrecord10.1016/j.virol.2022.06.004
rioxxterms.versionAM
dc.contributor.orcidFirth, Andrew [0000-0002-7986-9520]
dc.identifier.eissn1096-0341
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (106207/Z/14/Z)
pubs.funder-project-idWellcome Trust (220814/Z/20/Z)
cam.issuedOnline2022-06-05
cam.orpheus.success2022-06-10 - Embargo set during processing via Fast-track
cam.depositDate2022-06-06
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2023-06-05


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International