Reciprocal Best Structure Hits

In this work, we are using AlphaFold structure models to find the closest homologues proteins between Homo sapiens and D. melanogaster, C. elegans, S. cerevisiae and S. pombe as well as between S. cerevisiae and S. pombe. We are using the structure aligner Foldseek to run all against all and search for the best scoring hit in both directions to detect the Reciprocal Best Structure Hits (RBSH). We compare the results to protein pairs detected by their sequence similarity as Reciprocal Best Hits (RBH) and verify the results using the PANTHER family classification files. $$ \ $$ Note: This dataset is an earlier version of a more up-to-date dataset at https://doi.org/10.17863/CAM.87873

Format

We used the structure aligner Foldseek to find the RBSH as well as the sequence aligner BLASTP to find the RBH.

Keywords

AlphaFold database, Protein homology, Schizosaccharomyces pombe, Structure comparison

Relationships

Publication Reference: https://doi.org/10.1093/bioadv/vbac072 https://www.repository.cam.ac.uk/handle/1810/341511

Identifiers

This record's DOI: https://doi.org/10.17863/CAM.85487

Rights

Attribution 4.0 International (CC BY 4.0)

Licence URL: https://creativecommons.org/licenses/by/4.0/

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Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)