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dc.contributor.authorJiang, Haibin
dc.contributor.authorDu, Mingxia
dc.contributor.authorLi, Yaning
dc.contributor.authorZhou, Tengfei
dc.contributor.authorLei, Jia
dc.contributor.authorLiang, Hongqing
dc.contributor.authorZhong, Zhen
dc.contributor.authorAl-Lamki, Rafia
dc.contributor.authorJiang, Ming
dc.contributor.authorYang, Jun
dc.date.accessioned2022-06-15T15:01:12Z
dc.date.available2022-06-15T15:01:12Z
dc.date.issued2022-06-15
dc.date.submitted2021-12-03
dc.identifier.issn2041-4889
dc.identifier.others41419-022-04958-8
dc.identifier.other4958
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338122
dc.descriptionFunder: National Key Research and Development Program of China Stem Cell and Translational Research, 2016YFA0102300
dc.descriptionFunder: Zhejiang University Education Foundation Global Partnership Fund(188170+194452115/011)
dc.description.abstractInhibition of DNA binding proteins 1 and 3 (ID1 and ID3) are important downstream targets of BMP signalling that are necessary for embryonic development. However, their specific roles in regulating the pluripotency of human embryonic stem cells (hESCs) remain unclear. Here, we examined the roles of ID1 and ID3 in primed and naive-like hESCs and showed that ID1 and ID3 knockout lines (IDs KO) exhibited decreased survival in both primed and naive-like state. IDs KO lines in the primed state also tended to undergo pluripotent dissolution and ectodermal differentiation. IDs KO impeded the primed-to-naive transition (PNT) of hESCs, and overexpression of ID1 in primed hESCs promoted PNT. Furthermore, single-cell RNA sequencing demonstrated that ID1 and ID3 regulated the survival and pluripotency of hESCs through the AKT signalling pathway. Finally, we showed that TCF3 mediated transcriptional inhibition of MCL1 promotes AKT phosphorylation, which was confirmed by TCF3 knockdown in KO lines. Our study suggests that IDs/TCF3 acts through AKT signalling to promote survival and maintain pluripotency of both primed and naive-like hESCs.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectArticle
dc.subject/631/80/86/2364
dc.subject/631/532/2117
dc.subject/38
dc.subject/38/39
dc.subject/82
dc.subject/82/80
dc.subject/96
dc.subject/96/100
dc.subjectarticle
dc.titleID proteins promote the survival and primed-to-naive transition of human embryonic stem cells through TCF3-mediated transcription.
dc.typeArticle
dc.date.updated2022-06-15T15:01:11Z
prism.issueIdentifier6
prism.publicationNameCell Death Dis
prism.volume13
dc.identifier.doi10.17863/CAM.85531
dcterms.dateAccepted2022-05-16
rioxxterms.versionofrecord10.1038/s41419-022-04958-8
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidYang, Jun [0000-0001-9715-8100]
dc.identifier.eissn2041-4889
cam.issuedOnline2022-06-15


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