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dc.contributor.authorMazzitelli, Ignacio
dc.contributor.authorBleichmar, Lucía
dc.contributor.authorMelucci, Claudia
dc.contributor.authorGerber, Pehuén Pereyra
dc.contributor.authorToscanini, Agustina
dc.contributor.authorCuestas, María Luján
dc.contributor.authorDiaz, Fernando Erra
dc.contributor.authorGeffner, Jorge
dc.date.accessioned2022-06-17T09:00:07Z
dc.date.available2022-06-17T09:00:07Z
dc.date.issued2022
dc.date.submitted2021-12-09
dc.identifier.issn1664-3224
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338194
dc.description.abstractHigh salt (NaCl) concentrations are found in a number of tissues under physiological and pathological conditions. Here, we analyzed the effects induced by high salt on the function of human neutrophils. The culture of neutrophils in medium supplemented with high salt (50 mM NaCl) for short periods (30-120 min) inhibited the ability of conventional agonists to induce the production of IL-8 and the activation of respiratory burst. By contrast, exposure to high salt for longer periods (6-18 h) resulted in the activation of neutrophils revealed by the production of high levels of IL-8, the activation of the respiratory burst, and a marked synergistic effect on the production of TNF-α induced by LPS. Increasing osmolarity of the culture medium by the addition of sorbitol or mannitol (100 mM) was shown to be completely unable to stimulate neutrophil responses, suggesting that high sodium but not an increased osmolarity mediates the activation on neutrophils responses. A similar biphasic effect was observed when the function of monocytes was analyzed. Short term exposure to high salt suppressed IL-8 and TNF-α production induced by LPS while culture for longer periods triggered the production of IL-8 but not TNF-α in the absence of LPS stimulation. Contradictory results have been published regarding how high salt modulates neutrophil function. Our results suggest that the modulation of neutrophil function by high salt is strongly dependent on the exposure time.
dc.languageen
dc.publisherFrontiers Media SA
dc.subjectImmunology
dc.subjectinflammation
dc.subjectIL-8 (CXCL8)
dc.subjectoxygen reactive intermediates
dc.subjectsodium chloride
dc.subjectinnate immunity
dc.titleHigh Salt Induces a Delayed Activation of Human Neutrophils.
dc.typeArticle
dc.date.updated2022-06-17T09:00:06Z
prism.publicationNameFront Immunol
prism.volume13
dc.identifier.doi10.17863/CAM.85605
dcterms.dateAccepted2022-05-11
rioxxterms.versionofrecord10.3389/fimmu.2022.831844
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn1664-3224
cam.issuedOnline2022-06-03


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