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dc.contributor.authorMalkowska, Anna
dc.contributor.authorPenfold, Christopher
dc.contributor.authorBergmann, Sophie
dc.contributor.authorBoroviak, Thorsten E
dc.date.accessioned2022-06-17T16:01:24Z
dc.date.available2022-06-17T16:01:24Z
dc.date.issued2022-06-16
dc.date.submitted2021-02-04
dc.identifier.others41467-022-30194-x
dc.identifier.other30194
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338212
dc.description.abstractAbstract: Mammalian embryogenesis relies on glycolysis and oxidative phosphorylation to balance the generation of biomass with energy production. However, the dynamics of metabolic regulation in the postimplantation embryo in vivo have remained elusive due to the inaccessibility of the implanted conceptus for biochemical studies. To address this issue, we compiled single-cell embryo profiling data in six mammalian species and determined their metabolic dynamics through glycolysis and oxidative phosphorylation associated gene expression. Strikingly, we identify a conserved switch from bivalent respiration in the late blastocyst towards a glycolytic metabolism in early gastrulation stages across species, which is independent of embryo implantation. Extraembryonic lineages followed the dynamics of the embryonic lineage, except visceral endoderm. Finally, we demonstrate that in vitro primate embryo culture substantially impacts metabolic gene regulation by comparison to in vivo samples. Our work reveals a conserved metabolic programme despite different implantation modes and highlights the need to optimise postimplantation embryo culture protocols.
dc.languageen
dc.publisherNature Publishing Group UK
dc.subjectArticle
dc.subject/631/61/320
dc.subject/631/114/2114
dc.subject/631/136/2086/1986
dc.subject/631/443/319
dc.subject/631/1647/514/1949
dc.subject/38
dc.subject/38/91
dc.subject/45
dc.subjectarticle
dc.titleA hexa-species transcriptome atlas of mammalian embryogenesis delineates metabolic regulation across three different implantation modes
dc.typeArticle
dc.date.updated2022-06-17T16:01:23Z
prism.issueIdentifier1
prism.publicationNameNature Communications
prism.volume13
dc.identifier.doi10.17863/CAM.85623
dcterms.dateAccepted2022-04-21
rioxxterms.versionofrecord10.1038/s41467-022-30194-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidMalkowska, Anna [0000-0002-6195-0099]
dc.contributor.orcidPenfold, Christopher [0000-0001-5823-4705]
dc.contributor.orcidBoroviak, Thorsten E. [0000-0001-8703-8949]
dc.identifier.eissn2041-1723
pubs.funder-project-idIsaac Newton Trust (Isaac Newton Trust grant 19.07(j))
pubs.funder-project-idWellcome Trust (Wellcome) (RG89228)


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