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dc.contributor.authorRiding, Alexandra M
dc.contributor.authorLoudon, Kevin W
dc.contributor.authorGuo, Andrew
dc.contributor.authorFerdinand, John R
dc.contributor.authorLok, Laurence SC
dc.contributor.authorRichoz, Nathan
dc.contributor.authorStewart, Andrew
dc.contributor.authorCastro-Dopico, Tomas
dc.contributor.authorTuong, Zewen Kelvin
dc.contributor.authorFiancette, Remi
dc.contributor.authorBowyer, Georgina S
dc.contributor.authorFleming, Aaron
dc.contributor.authorGillman, Eleanor S
dc.contributor.authorSuchanek, Ondrej
dc.contributor.authorMahbubani, Krishnaa T
dc.contributor.authorSaeb-Parsy, Kourosh
dc.contributor.authorWithers, David
dc.contributor.authorDougan, Gordan
dc.contributor.authorClare, Simon
dc.contributor.authorClatworthy, Menna R
dc.date.accessioned2022-06-25T23:30:06Z
dc.date.available2022-06-25T23:30:06Z
dc.date.issued2022-07-15
dc.identifier.issn2589-0042
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338367
dc.description.abstractBladder infection affects a hundred million people annually, but our understanding of bladder immunity is incomplete. We found type 17 immune response genes among the most up-regulated networks in mouse bladder following uropathogenic Escherichia coli (UPEC) challenge. Intravital imaging revealed submucosal Rorc+ cells responsive to UPEC challenge, and we found increased Il17 and IL22 transcripts in wild-type and Rag2 -/- mice, implicating group 3 innate lymphoid cells (ILC3s) as a source of these cytokines. NCR-positive and negative ILC3 subsets were identified in murine and human bladders, with local proliferation increasing IL17-producing ILC3s post infection. ILC3s made a more limited contribution to bladder IL22, with prominent early induction of IL22 evident in Th17 cells. Single-cell RNA sequencing revealed bladder NCR-negative ILC3s as the source of IL17 and identified putative ILC3-myeloid cell interactions, including via lymphotoxin-β-LTBR. Altogether, our data provide important insights into the orchestration and execution of type 17 immunity in bladder defense.
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleGroup 3 innate lymphocytes make a distinct contribution to type 17 immunity in bladder defence.
dc.typeArticle
dc.publisher.departmentDepartment of Medicine
dc.date.updated2022-06-24T10:47:34Z
prism.endingPage104660
prism.number104660
prism.publicationDate2022
prism.publicationNameiScience
prism.startingPage104660
dc.identifier.doi10.17863/CAM.85776
dcterms.dateAccepted2022-06-16
rioxxterms.versionofrecord10.1016/j.isci.2022.104660
rioxxterms.versionVoR
dc.contributor.orcidTuong, Kelvin [0000-0002-6735-6808]
dc.contributor.orcidDougan, Gordon [0000-0003-0022-965X]
dc.contributor.orcidClatworthy, Menna [0000-0002-3340-9828]
dc.identifier.eissn2589-0042
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (200110/Z/15/Z)
pubs.funder-project-idWellcome Trust (220268/Z/20/Z)
pubs.funder-project-idKidney Research UK (TF_013_20171124)
pubs.funder-project-idWellcome Trust (106809/Z/15/Z)
pubs.funder-project-idMedical Research Council (MR/N024907/1)
pubs.funder-project-idArthritis Research UK (21777)
cam.issuedOnline2022-06-22
cam.orpheus.success2022-06-25 - Embargo set during processing via Fast-track
cam.depositDate2022-06-24
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2022-06-30


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International