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dc.contributor.authorRöder, Konstantin
dc.contributor.authorBarker, Amy M
dc.contributor.authorWhitehouse, Adrian
dc.contributor.authorPasquali, Samuela
dc.date.accessioned2022-06-29T19:48:03Z
dc.date.available2022-06-29T19:48:03Z
dc.date.issued2022-05
dc.identifier.issn1553-734X
dc.identifier.other35617364
dc.identifier.otherPMC9176763
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338555
dc.descriptionFunder: Cambridge Philosophical Society
dc.descriptionFunder: Universite de Paris
dc.description.abstractKaposi's sarcoma-associated herpes virus (KSHV) is a human oncovirus. KSHV relies on manipulating the host cell N6-methyl adenosine (m6A) RNA modification pathway to enhance virus replication. Methylation within a RNA stem loop of the open reading frame 50 (ORF50) increases transcript stability via the recruitment of the m6A reader, SND1. In this contribution we explore the energy landscapes of the unmethylated and methylated RNA stem loops of ORF50 to investigate the effect of methylation on the structure of the stem loop. We observe a significant shift upon methylation between an open and closed configuration of the top of the stem loop. In the unmethylated stem loop the closed configuration is much lower in energy, and, as a result, exhibits higher occupancy.
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1553-7358
dc.sourcenlmid: 101238922
dc.subjectCell Line
dc.subjectHumans
dc.subjectHerpesvirus 8, Human
dc.subjectSarcoma, Kaposi
dc.subjectEndonucleases
dc.subjectRNA
dc.subjectAdenosine
dc.subjectGene Expression Regulation, Viral
dc.subjectMethylation
dc.subjectOpen Reading Frames
dc.titleInvestigating the structural changes due to adenosine methylation of the Kaposi's sarcoma-associated herpes virus ORF50 transcript.
dc.typeArticle
dc.date.updated2022-06-29T19:48:03Z
prism.issueIdentifier5
prism.publicationNamePLoS Comput Biol
prism.volume18
dc.identifier.doi10.17863/CAM.85968
dcterms.dateAccepted2022-04-28
rioxxterms.versionofrecord10.1371/journal.pcbi.1010150
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidRöder, Konstantin [0000-0003-2021-9504]
dc.contributor.orcidBarker, Amy M [0000-0003-1626-0934]
dc.contributor.orcidWhitehouse, Adrian [0000-0003-3866-7110]
dc.contributor.orcidPasquali, Samuela [0000-0003-1487-0894]
dc.identifier.eissn1553-7358
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/V006363/1, BB/T00021X/1)
pubs.funder-project-idMedical Research Foundation (MR/R010145/1)
pubs.funder-project-idMedical Research Council (MR/R010145/1)
cam.issuedOnline2022-05-26


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International