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dc.contributor.authorClark, Bethan
dc.contributor.authorElkin, Joel
dc.contributor.authorMarconi, Aleksandra
dc.contributor.authorTurner, George F
dc.contributor.authorSmith, Alan M
dc.contributor.authorJoyce, Domino
dc.contributor.authorMiska, Eric A
dc.contributor.authorJuntti, Scott A
dc.contributor.authorSantos, M Emília
dc.date.accessioned2022-06-29T19:48:33Z
dc.date.available2022-06-29T19:48:33Z
dc.date.issued2022-04
dc.identifier.issn2054-5703
dc.identifier.other35601449
dc.identifier.otherPMC9019512
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338566
dc.description.abstractIdentifying genetic loci underlying trait variation provides insights into the mechanisms of diversification, but demonstrating causality and characterizing the role of genetic loci requires testing candidate gene function, often in non-model species. Here we establish CRISPR/Cas9 editing in Astatotilapia calliptera, a generalist cichlid of the remarkably diverse Lake Malawi radiation. By targeting the gene oca2 required for melanin synthesis in other vertebrate species, we show efficient editing and germline transmission. Gene edits include indels in the coding region, probably a result of non-homologous end joining, and a large deletion in the 3' untranslated region due to homology-directed repair. We find that oca2 knock-out A. calliptera lack melanin, which may be useful for developmental imaging in embryos and studying colour pattern formation in adults. As A. calliptera resembles the presumed generalist ancestor of the Lake Malawi cichlids radiation, establishing genome editing in this species will facilitate investigating speciation, adaptation and trait diversification in this textbook radiation.
dc.languageeng
dc.publisherThe Royal Society
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2054-5703
dc.sourcenlmid: 101647528
dc.subjectAdaptive radiation
dc.subjectEvo-devo
dc.subjectFunctional Genetics
dc.subjectCichlids
dc.subjectCrispr/cas9
dc.titleOca2 targeting using CRISPR/Cas9 in the Malawi cichlid Astatotilapia calliptera.
dc.typeArticle
dc.date.updated2022-06-29T19:48:33Z
prism.issueIdentifier4
prism.publicationNameR Soc Open Sci
prism.volume9
dc.identifier.doi10.17863/CAM.85979
dcterms.dateAccepted2022-03-22
rioxxterms.versionofrecord10.1098/rsos.220077
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidJoyce, Domino [0000-0002-4253-5623]
dc.contributor.orcidSantos, M Emília [0000-0003-3158-7935]
dc.identifier.eissn2054-5703
pubs.funder-project-idCancer Research UK (C13474/A18583, C6946/A14492)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z, 222279/Z/20/Z, 219475/Z/19/Z, 102175/Z/13/Z)
pubs.funder-project-idNSF (IOS-1825723)
pubs.funder-project-idHuman Frontier Science Program (RGY0079/2018)
pubs.funder-project-idNatural Environment Research Council (NE/R01504X/1)
cam.issuedOnline2022-04-20


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International