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dc.contributor.authorvan de Kerkhof, Gea T
dc.contributor.authorSchertel, Lukas
dc.contributor.authorCatòn, Laura
dc.contributor.authorParton, Thomas G
dc.contributor.authorMüller, Karin H
dc.contributor.authorGreer, Heather F
dc.contributor.authorIngham, Colin J
dc.contributor.authorVignolini, Silvia
dc.date.accessioned2022-06-29T19:48:50Z
dc.date.available2022-06-29T19:48:50Z
dc.date.issued2022-05
dc.identifier.citationJournal of the Royal Society, Interface, volume 19, issue 190, page 20220181
dc.identifier.issn1742-5689
dc.identifier.other35611622
dc.identifier.otherPMC9131120
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338573
dc.description.abstractThe brightest colours in nature often originate from the interaction of light with materials structured at the nanoscale. Different organisms produce such coloration with a wide variety of materials and architectures. In the case of bacterial colonies, structural colours stem for the periodic organization of the cells within the colony, and while considerable efforts have been spent on elucidating the mechanisms responsible for such coloration, the biochemical processes determining the development of this effect have not been explored. Here, we study the influence of nutrients on the organization of cells from the structurally coloured bacteria Flavobacterium strain IR1. By analysing the optical properties of the colonies grown with and without specific polysaccharides, we found that the highly ordered organization of the cells can be altered by the presence of fucoidans. Additionally, by comparing the organization of the wild-type strain with mutants grown in different nutrient conditions, we deduced that this regulation of cell ordering is linked to a specific region of the IR1 chromosome. This region encodes a mechanism for the uptake and metabolism of polysaccharides, including a polysaccharide utilization locus (PUL operon) that appears specific to fucoidan, providing new insight into the biochemical pathways regulating structural colour in bacteria.
dc.description.sponsorshipThis work was funded by by EU’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 722842 (ITN Plant-inspired Materials and Surfaces–PlaMatSu), the Swiss National Science Foundation under project P2ZHP2_183998, the Biotechnology and Biological Sciences Research Council (BBSRC) iCASE fellowship (2110570), the BBSRC David Phillips fellowship (BB/K014617/1), the BBSRC grant BB/V00364X/1, the ERC (ERC-St-Grant014H2020 639088), the ERC BiTe (101001637) and the Engineering, the Physical Sciences Research Council (ESPRC) NanoDTC grant (EP/L015978/1) and the EPSRC Underpinning Multi-User Equipment Call (EP/P030467/1). CI would like to thank the iLAB, Utrecht, for facilities.
dc.languageeng
dc.publisherThe Royal Society
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1742-5662
dc.sourcenlmid: 101217269
dc.subjectPolysaccharides
dc.subjectMetabolism
dc.subjectRegulation
dc.subjectFucoidan
dc.subjectBiochemical Pathway
dc.subjectStructurally Coloured Bacteria
dc.subjectBacteria
dc.subjectColor
dc.titlePolysaccharide metabolism regulates structural colour in bacterial colonies.
dc.typeArticle
dc.date.updated2022-06-29T19:48:50Z
prism.publicationNameJ R Soc Interface
dc.identifier.doi10.17863/CAM.85986
dcterms.dateAccepted2022-04-01
rioxxterms.versionofrecord10.1098/rsif.2022.0181
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidvan de Kerkhof, Gea T [0000-0003-2427-2740]
dc.contributor.orcidSchertel, Lukas [0000-0003-0977-0389]
dc.contributor.orcidCatòn, Laura [0000-0003-2905-3045]
dc.contributor.orcidParton, Thomas G [0000-0001-7153-1042]
dc.contributor.orcidMüller, Karin H [0000-0003-4693-8558]
dc.contributor.orcidGreer, Heather F [0000-0001-6149-1964]
dc.contributor.orcidIngham, Colin J [0000-0003-1508-019X]
dc.contributor.orcidVignolini, Silvia [0000-0003-0664-1418]
dc.identifier.eissn1742-5662
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (722842)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (2110570)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/K014617/1)
pubs.funder-project-idBBSRC (BB/V00364X/1)
pubs.funder-project-idEuropean Research Council (639088)
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) ERC (101001637)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/P030467/1)
cam.issuedOnline2022-05-25


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International