Production of <i>Purpureocillium lilacinum</i> and <i>Pochonia chlamydosporia</i> by Submerged Liquid Fermentation and Bioactivity against <i>Tetranychus urticae</i> and <i>Heterodera glycines</i> through Seed Inoculation.
View / Open Files
Authors
Mascarin, Gabriel Moura
Publication Date
2022-05-16ISSN
2309-608X
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Silva, D. M., de Souza, V. H. M., Moral, R. d. A., Delalibera Júnior, I., & Mascarin, G. M. (2022). Production of <i>Purpureocillium lilacinum</i> and <i>Pochonia chlamydosporia</i> by Submerged Liquid Fermentation and Bioactivity against <i>Tetranychus urticae</i> and <i>Heterodera glycines</i> through Seed Inoculation.. https://doi.org/10.3390/jof8050511
Abstract
<i>Pochonia</i><i>chlamydosporia</i> and <i>Purpureocillium</i><i>lilacinum</i> are fungal bioagents used for the sustainable management of plant parasitic nematodes. However, their production through submerged liquid fermentation and their use in seed treatment have been underexplored. Therefore, our goal was to assess the effect of different liquid media on the growth of 40 isolates of <i>P</i>. <i>lilacinum</i> and two of <i>P</i>. <i>chlamydosporia</i>. The most promising isolates tested were assessed for plant growth promotion and the control of the two-spotted spider mite (<i>Tetranychus urticae</i>) and the soybean cyst nematode (<i>Heterodera glycines</i>). Most isolates produced > 10<sup>8</sup> blastospores mL<sup>-1</sup> and some isolates produced more than 10<sup>4</sup> microsclerotia mL<sup>-1</sup>. Microsclerotia of selected isolates were used to inoculate common bean (<i>Phaseolus vulgaris</i> L.) seeds in greenhouse trials. All fungal isolates reduced the <i>T. urticae</i> fecundity in inoculated plants through seed treatment, while <i>P. chlamydosporia</i> ESALQ5406 and <i>P. lilacinum</i> ESALQ2593 decreased cyst nematode population. <i>Purpureocillium lilacinum</i> was more frequently detected in soil, whereas <i>P. chlamydosporia</i> colonized all plant parts. <i>Pochonia chlamydosporia</i> ESALQ5406 improved the root development of bean plants. These findings demonstrate the possibility of producing submerged propagules of <i>P. chlamydosporia</i> and <i>P. lilacinum</i> by liquid culture, and greenhouse trials support the applicability of fungal microsclerotia in seed treatment to control <i>P. vulgaris</i> pests.
Keywords
Biological control, soybean cyst nematode, Seed treatment, Microsclerotia, Two-spotted Spider Mite
Identifiers
35628766, PMC9145524
External DOI: https://doi.org/10.3390/jof8050511
This record's URL: https://www.repository.cam.ac.uk/handle/1810/338577
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.