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dc.contributor.authorRudova, Nataliia
dc.contributor.authorButtler, Jeremy
dc.contributor.authorKovalenko, Ganna
dc.contributor.authorSushko, Mykola
dc.contributor.authorBolotin, Vitaliy
dc.contributor.authorMuzykina, Larysa
dc.contributor.authorZinenko, Oleksandr
dc.contributor.authorStegniy, Borys
dc.contributor.authorDunaiev, Yurii
dc.contributor.authorSytiuk, Mykola
dc.contributor.authorGerilovych, Anton
dc.contributor.authorDrown, Devin M
dc.contributor.authorBortz, Eric
dc.contributor.authorSolodiankin, Oleksii
dc.date.accessioned2022-06-29T19:49:06Z
dc.date.available2022-06-29T19:49:06Z
dc.date.issued2022-04-28
dc.identifier.issn1999-4915
dc.identifier.other35632666
dc.identifier.otherPMC9142977
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338579
dc.description.abstractPorcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For a non-enveloped virus with a small genome (1.7 kb ssDNA), PCV2 is remarkably diverse, with eight genotypes (a-h). New genotypes of PCV2 can spread through the migration of wild boar, which are thought to infect domestic pigs and spread further through the domestic pig trade. Despite a large swine population, the diversity of PCV2 genotypes in Ukraine has been under-sampled, with few PCV2 genome sequences reported in the past decade. To gain a deeper understanding of PCV2 genotype diversity in Ukraine, samples of blood serum were collected from wild boars (n = 107) that were hunted in Ukraine during the November-December 2012 hunting season. We found 34/107 (31.8%) prevalence of PCV2 by diagnostic PCR. For domestic pigs, liver samples (n = 16) were collected from a commercial market near Kharkiv in 2019, of which 6 out of 16 (37%) samples were positive for PCV2. We sequenced the genotyping locus ORF2, a gene encoding the PCV2 viral capsid (Cap), for 11 wild boar and six domestic pig samples in Ukraine using an Oxford Nanopore MinION device. Of 17 samples with resolved genotypes, the PCV2 genotype b was the most common in wild boar samples (10 out of 11, 91%), while the domestic pigs were infected with genotypes b and d. We also detected genotype b/d and b/a co-infections in wild boars and domestic pigs, respectively, and for the first time in Ukraine we detected genotype f in a wild boar from Poltava. Building a maximum-likelihood phylogeny, we identified a sublineage of PCV2 genotype b infections in both wild and domestic swine, suggesting a possible epizootic cluster and an ecological interaction between wild boar and domestic pig populations in northeastern Ukraine.
dc.languageeng
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 1999-4915
dc.sourcenlmid: 101509722
dc.subjectGenotype
dc.subjectDomestic pig
dc.subjectPorcine circovirus
dc.subjectWild boar
dc.subjectPCV2
dc.subjectphylogenetics
dc.subjectUkraine
dc.subjectMinion
dc.subjectAnimals
dc.subjectSwine
dc.subjectSus scrofa
dc.subjectCircovirus
dc.subjectSwine Diseases
dc.subjectGenetic Variation
dc.titleGenetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine.
dc.typeArticle
dc.date.updated2022-06-29T19:49:06Z
prism.issueIdentifier5
prism.publicationNameViruses
prism.volume14
dc.identifier.doi10.17863/CAM.85992
dcterms.dateAccepted2022-04-22
rioxxterms.versionofrecord10.3390/v14050924
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidKovalenko, Ganna [0000-0002-4929-0841]
dc.contributor.orcidZinenko, Oleksandr [0000-0001-5228-9940]
dc.contributor.orcidDrown, Devin M [0000-0002-2437-7019]
dc.contributor.orcidBortz, Eric [0000-0002-1376-0012]
dc.identifier.eissn1999-4915
pubs.funder-project-idNIGMS NIH HHS (2P20GM103395)
cam.issuedOnline2022-04-28


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International