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dc.contributor.authorZaidan, Nada
dc.contributor.authorNitsche, Leslie
dc.contributor.authorDiamanti, Evangelia
dc.contributor.authorHannah, Rebecca
dc.contributor.authorFidanza, Antonella
dc.contributor.authorWilson, Nicola K
dc.contributor.authorForrester, Lesley M
dc.contributor.authorGöttgens, Berthold
dc.contributor.authorOttersbach, Katrin
dc.date.accessioned2022-07-04T23:30:55Z
dc.date.available2022-07-04T23:30:55Z
dc.date.issued2022-08-09
dc.identifier.issn2213-6711
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338759
dc.description.abstractTo generate sufficient numbers of transplantable hematopoietic stem cells (HSCs) in vitro, a detailed understanding of how this process takes place in vivo is essential. The endothelial-to-hematopoietic transition (EHT), which culminates in the production of the first HSCs, is a highly complex process during which key regulators are switched on and off at precise moments, and that is embedded into a myriad of microenvironmental signals from surrounding cells and tissues. We have previously demonstrated an HSC-supportive function for GATA3 within the sympathetic nervous system and the sub-aortic mesenchyme, but show here that it also plays a cell-intrinsic role during the EHT. It is expressed in hemogenic endothelial cells and early HSC precursors, where its expression correlates with a more quiescent state. Importantly, endothelial-specific deletion of Gata3 shows that it is functionally required for these cells to mature into HSCs, placing GATA3 at the core of the EHT regulatory network.
dc.publisherElsevier BV
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCambridge Stem Cell Institute
dc.titleEndothelial-specific Gata3 expression is required for hematopoietic stem cell generation.
dc.typeArticle
dc.publisher.departmentDepartment of Haematology
dc.date.updated2022-07-04T12:47:44Z
prism.publicationNameStem Cell Reports
dc.identifier.doi10.17863/CAM.86169
dcterms.dateAccepted2022-06-27
rioxxterms.versionofrecord10.1016/j.stemcr.2022.06.008
rioxxterms.versionAM
dc.contributor.orcidGottgens, Berthold [0000-0001-6302-5705]
dc.identifier.eissn2213-6711
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (203151/Z/16/Z)
pubs.funder-project-idWellcome Trust (203151/A/16/Z)
cam.issuedOnline2022-07-28
cam.orpheus.successWed Aug 03 09:46:02 BST 2022 - This item is covered by RRS with an embargo. The item is now published and embargo has been lifted.
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cam.depositDate2022-07-04
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International