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dc.contributor.authorMcDonald, Jennifer
dc.contributor.authorMerrick, Catherine
dc.date.accessioned2022-07-05T20:00:12Z
dc.date.available2022-07-05T20:00:12Z
dc.date.issued2022-06
dc.date.submitted2021-12-20
dc.identifier.citationPLOS Pathogens, volume 18, issue 6, article-number e1010595
dc.identifier.issn1553-7366
dc.identifier.otherppathogens-d-21-02568
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338820
dc.description.abstractMalaria parasites are unusual, early-diverging protozoans with non-canonical cell cycles. They do not undergo binary fission, but divide primarily by schizogony. This involves the asynchronous production of multiple nuclei within the same cytoplasm, culminating in a single mass cytokinesis event. The rate and efficiency of parasite reproduction is fundamentally important to malarial disease, which tends to be severe in hosts with high parasite loads. Here, we have studied for the first time the dynamics of schizogony in two human malaria parasite species, Plasmodium falciparum and Plasmodium knowlesi. These differ in their cell-cycle length, the number of progeny produced and the genome composition, among other factors. Comparing them could therefore yield new information about the parameters and limitations of schizogony. We report that the dynamics of schizogony differ significantly between these two species, most strikingly in the gap phases between successive nuclear multiplications, which are longer in P. falciparum and shorter, but more heterogenous, in P. knowlesi. In both species, gaps become longer as schizogony progresses, whereas each period of active DNA replication grows shorter. In both species there is also extreme variability between individual cells, with some schizonts producing many more nuclei than others, and some individual nuclei arresting their DNA replication for many hours while adjacent nuclei continue to replicate. The efficiency of schizogony is probably influenced by a complex set of factors in both the parasite and its host cell.
dc.languageen
dc.publisherPublic Library of Science (PLoS)
dc.subjectResearch Article
dc.subjectBiology and life sciences
dc.subjectResearch and analysis methods
dc.subjectMedicine and health sciences
dc.titleDNA replication dynamics during erythrocytic schizogony in the malaria parasites Plasmodium falciparum and Plasmodium knowlesi.
dc.typeArticle
dc.date.updated2022-07-05T20:00:11Z
prism.publicationNamePLoS Pathog
dc.identifier.doi10.17863/CAM.86227
dcterms.dateAccepted2022-05-13
rioxxterms.versionofrecord10.1371/journal.ppat.1010595
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisoreditor: Blackman, Michael J.
dc.contributor.orcidMerrick, Catherine [0000-0001-7583-2176]
dc.identifier.eissn1553-7374
pubs.funder-project-idEuropean Research Council (725126)
cam.issuedOnline2022-06-22


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