Research data supporting: "Dysregulation of ADAM10 shedding activity in naked mole-rat fibroblasts is due to deficient phosphatidylserine externalisation"
Citation
Smith, E. (2023). Research data supporting: "Dysregulation of ADAM10 shedding activity in naked mole-rat fibroblasts is due to deficient phosphatidylserine externalisation" [Dataset]. https://doi.org/10.17863/CAM.85571
Description
There is a Prism file providing the raw data, including statistical analyses, aligning to each of the figures:
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Figure 1.pzfx - CD44 cleavage assay in mouse and naked mole-rat primary skin fibroblasts
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Figure 2.pzfx - Measurement of cellular ADAM10 levels and betacellulin cleavage in mouse and naked mole-rat primary skin fibroblasts
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Figure 3.pzfx - CD44 and betacellulin cleavage assays in mouse and naked mole-rat transformed skin fibroblasts
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Figure 4.pzfx - Plasma membrane phosphatidlylserine quantification in mouse and naked mole-rat primary skin fibroblasts
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Figure 5.pzfx - Analysis of plasma membrane phosphatidlylserine levels and betacellulin cleavage in mouse and naked mole-rat primary skin fibroblasts overexpressing either GFP or the scramblase ANO6
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Figure 6.pzfx - Analysis of cellular wound closure in naked mole-rat primary skin fibroblasts overexpressing either GFP or the scramblase ANO6, and the impact of inhibiting ADAM10
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Supplementary Figure 1.pzfx - Concentration response relationship for ionomycin induced CD44 cleavage in mouse and naked mole-rat primary skin fibroblasts
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Supplementary Figure 2.pzfx - Concentration response relationship for ionomycin induced CD44 cleavage in transformed mouse and naked mole-rat skin fibroblasts
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Supplementary Figure 3.pzfx - Levels of cellular ADAM10 levels in mouse and naked mole-rat transformed skin fibroblasts
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Supplementary Figure 4.pzfx - Analysis of plasma membrane phosphatidlylserine levels and betacellulin cleavage in mouse primary skin fibroblasts overexpressing either GFP or the scramblase ANO6, carried out at 32°C
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Supplementary Figure 5.pzfx - Analysis of ANO6 protein levels in mouse and naked mole-rat transformed fibroblasts
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Supplementary Figure 6.pzfx - Analysis of cellular wound closure in mouse primary skin fibroblasts overexpressing either GFP or the scramblase ANO6, and showing the impact of ADAM10 inhibition, carried out at 32°C and 37°C
Format
All files open with Graphpad Prism
Keywords
Cell growth, Western blot, Wound closure
Relationships
Related research output: https://doi.org/10.1002/jcp.30972
Sponsorship
Dunhill Medical Trust (RPGF2002\188)
Funder references
Dunhill Medical Trust (RPGF2002\188)
Identifiers
This record's DOI: https://doi.org/10.17863/CAM.85571
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/
Statistics
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IRUS guide.
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