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Research data supporting: "Dysregulation of ADAM10 shedding activity in naked mole-rat fibroblasts is due to deficient phosphatidylserine externalisation"


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Description

There is a Prism file providing the raw data, including statistical analyses, aligning to each of the figures:

 

Figure 1.pzfx - CD44 cleavage assay in mouse and naked mole-rat primary skin fibroblasts

 

Figure 2.pzfx - Measurement of cellular ADAM10 levels and betacellulin cleavage in mouse and naked mole-rat primary skin fibroblasts

 

Figure 3.pzfx - CD44 and betacellulin cleavage assays in mouse and naked mole-rat transformed skin fibroblasts

 

Figure 4.pzfx - Plasma membrane phosphatidlylserine quantification in mouse and naked mole-rat primary skin fibroblasts

 

Figure 5.pzfx - Analysis of plasma membrane phosphatidlylserine levels and betacellulin cleavage in mouse and naked mole-rat primary skin fibroblasts overexpressing either GFP or the scramblase ANO6

 

Figure 6.pzfx - Analysis of cellular wound closure in naked mole-rat primary skin fibroblasts overexpressing either GFP or the scramblase ANO6, and the impact of inhibiting ADAM10

 

Supplementary Figure 1.pzfx - Concentration response relationship for ionomycin induced CD44 cleavage in mouse and naked mole-rat primary skin fibroblasts

 

Supplementary Figure 2.pzfx - Concentration response relationship for ionomycin induced CD44 cleavage in transformed mouse and naked mole-rat skin fibroblasts

 

Supplementary Figure 3.pzfx - Levels of cellular ADAM10 levels in mouse and naked mole-rat transformed skin fibroblasts

 

Supplementary Figure 4.pzfx - Analysis of plasma membrane phosphatidlylserine levels and betacellulin cleavage in mouse primary skin fibroblasts overexpressing either GFP or the scramblase ANO6, carried out at 32°C

 

Supplementary Figure 5.pzfx - Analysis of ANO6 protein levels in mouse and naked mole-rat transformed fibroblasts

 

Supplementary Figure 6.pzfx - Analysis of cellular wound closure in mouse primary skin fibroblasts overexpressing either GFP or the scramblase ANO6, and showing the impact of ADAM10 inhibition, carried out at 32°C and 37°C

Version

Software / Usage instructions

All files open with Graphpad Prism

Keywords

Cell growth, Western blot, Wound closure

Publisher

Sponsorship
Dunhill Medical Trust (RPGF2002\188)
Dunhill Medical Trust (RPGF2002\188)
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