Reconstructing Gene Regulatory Networks That Control Hematopoietic Commitment.

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Hamey, Fiona K 
Göttgens, Berthold 

Hematopoietic stem cells (HSCs) reside at the apex of the hematopoietic hierarchy, possessing the ability to self-renew and differentiate toward all mature blood lineages. Along with more specialized progenitor cells, HSCs have an essential role in maintaining a healthy blood system. Incorrect regulation of cell fate decisions in stem/progenitor cells can lead to an imbalance of mature blood cell populations-a situation seen in diseases such as leukemia. Transcription factors, acting as part of complex regulatory networks, are known to play an important role in regulating hematopoietic cell fate decisions. Yet, discovering the interactions present in these networks remains a big challenge. Here, we discuss a computational method that uses single-cell gene expression data to reconstruct Boolean gene regulatory network models and show how this technique can be applied to enhance our understanding of transcriptional regulation in hematopoiesis.

Boolean network, Gene regulatory network, Hematopoiesis, Hematopoietic stem/progenitor cell, Pseudotime, Single cell, Transcriptional regulation, Cell Differentiation, Cell Lineage, Computational Biology, Gene Regulatory Networks, Hematopoiesis, Hematopoietic Stem Cells, Humans, Transcriptome
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Methods Mol Biol
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Springer New York
Medical Research Council (MC_PC_12009)
Work in the author’s laboratory is supported by grants from the Wellcome, Bloodwise, Cancer Research UK, NIH-NIDDK and core support grants by the Wellcome to the Cambridge Institute for Medical Research and Wellcome & MRC Cambridge Stem Cell Institute. F.K.H. is a recipient of a Medical Research Council PhD Studentship.