Clamping of DNA shuts the condensin neck gate.

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Rhodes, James 

SignificanceDNA needs to be compacted to fit into nuclei and during cell division, when dense chromatids are formed for their mechanical segregation, a process that depends on the protein complex condensin. It forms and enlarges loops in DNA through loop extrusion. Our work resolves the atomic structure of a DNA-bound state of condensin in which ATP has not been hydrolyzed. The DNA is clamped within a compartment that has been reported previously in other structural maintenance of chromosomes (SMC) complexes, including Rad50, cohesin, and MukBEF. With the caveat of important differences, it means that all SMC complexes cycle through at least some similar states and undergo similar conformational changes in their head modules, while hydrolyzing ATP and translocating DNA.

DNA motor, SMC complex, cryo-EM, loop extrusion, Adenosine Triphosphatases, Adenosine Triphosphate, Cell Cycle Proteins, Chromatids, Constriction, DNA, DNA-Binding Proteins, Multiprotein Complexes
Journal Title
Proc Natl Acad Sci U S A
Conference Name
Journal ISSN
Volume Title
Proceedings of the National Academy of Sciences
Wellcome (218621/Z/19/Z)
Wellcome (202754/Z/16/Z)
RCUK | Medical Research Council (MRC) (U105184326)