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Generation of a three-dimensional collagen scaffold-based model of the human endometrium.

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dc.contributor.authorAbbas, Yassen
dc.contributor.authorBrunel, Lucia G
dc.contributor.authorHollinshead, Michael S
dc.contributor.authorFernando, Ridma C
dc.contributor.authorGardner, Lucy
dc.contributor.authorDuncan, Imogen
dc.contributor.authorMoffett, Ashley
dc.contributor.authorBest, Serena
dc.contributor.authorTurco, Margherita Y
dc.contributor.authorBurton, Graham J
dc.contributor.authorCameron, Ruth E
dc.contributor.orcidAbbas, Yassen [0000-0001-8052-6691]
dc.contributor.orcidBrunel, Lucia G [0000-0003-0327-5635]
dc.date.accessioned2020-04-21T00:24:46Z
dc.date.available2020-04-21T00:24:46Z
dc.date.issued2020-02-14
dc.date.updated2020-04-21T00:24:45Z
dc.description.abstractThe endometrium is the secretory lining of the uterus that undergoes dynamic changes throughout the menstrual cycle in preparation for implantation and a pregnancy. Recently, endometrial organoids (EO) were established to study the glandular epithelium. We have built upon this advance and developed a multi-cellular model containing both endometrial stromal and epithelial cells. We use porous collagen scaffolds produced with controlled lyophilization to direct cellular organization, integrating organoids with primary isolates of stromal cells. The internal pore structure of the scaffold was optimized for stromal cell culture in a systematic study, finding an optimal average pore size of 101 µm. EO seeded organize to form a luminal-like epithelial layer, on the surface of the scaffold. The cells polarize with their apical surface carrying microvilli and cilia that face the pore cavities and their basal surface attaching to the scaffold with the formation of extracellular matrix proteins. Both cell types are hormone responsive on the scaffold, with hormone stimulation resulting in epithelial differentiation and stromal decidualization.
dc.identifier.doi10.17863/CAM.51652
dc.identifier.issn2042-8898
dc.identifier.otherPMC7061944
dc.identifier.other32194932
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304570
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2042-8901
dc.sourcenlmid: 101531990
dc.subjectEndometrium
dc.subjectCo-culture
dc.subjectOrganoids
dc.subjectCollagen Scaffolds
dc.titleGeneration of a three-dimensional collagen scaffold-based model of the human endometrium.
dc.typeArticle
prism.issueIdentifier2
prism.publicationNameInterface focus
prism.volume10
pubs.funder-project-idWellcome Trust (085992/Z/08/Z, 090108/Z/09/Z)
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1098/rsfs.2019.0079

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