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Tailored Mobility in a Zeolite Imidazolate Framework (ZIF) Antibody Conjugate*.

Published version
Peer-reviewed

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Abstract

Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates and the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved the integrity, stability, and dispersibility of the antibody conjugates, LC-MS/MS provided evidence that a covalent link was established between these metal-organic frameworks and lysine residues in IgG antibodies.

Description

Keywords

antibodies, immunoassays, metal-organic frameworks, nanoparticles, zeolite analogues, Chromatography, Liquid, Horseradish Peroxidase, Metal-Organic Frameworks, Tandem Mass Spectrometry, Zeolites

Journal Title

Chemistry

Conference Name

Journal ISSN

0947-6539
1521-3765

Volume Title

Publisher

Wiley