cam.issuedOnline	2018-11-16
dc.contributor.author	Fraslin, Clémence
dc.contributor.author	Dechamp, Nicolas
dc.contributor.author	Bernard, Maria
dc.contributor.author	Krieg, Francine
dc.contributor.author	Hervet, Caroline
dc.contributor.author	Guyomard, René
dc.contributor.author	Esquerré, Diane
dc.contributor.author	Barbieri, Johanna
dc.contributor.author	Kuchly, Claire
dc.contributor.author	Duchaud, Eric
dc.contributor.author	Boudinot, Pierre
dc.contributor.author	Rochat, Tatiana
dc.contributor.author	Bernardet, Jean-François
dc.contributor.author	Quillet, Edwige
dc.date.accessioned	2018-11-17T07:03:51Z
dc.date.available	2018-11-17T07:03:51Z
dc.date.issued	2018-11-16
dc.date.updated	2018-11-17T07:03:49Z
dc.description.abstract	BACKGROUND: Bacterial cold-water disease, which is caused by Flavobacterium psychrophilum, is one of the major diseases that affect rainbow trout (Oncorhynchus mykiss) and a primary concern for trout farming. Better knowledge of the genetic basis of resistance to F. psychrophilum would help to implement this trait in selection schemes and to investigate the immune mechanisms associated with resistance. Various studies have revealed that skin and mucus may contribute to response to infection. However, previous quantitative trait loci (QTL) studies were conducted by using injection as the route of infection. Immersion challenge, which is assumed to mimic natural infection by F. psychrophilum more closely, may reveal different defence mechanisms. RESULTS: Two isogenic lines of rainbow trout with contrasting susceptibilities to F. psychrophilum were crossed to produce doubled haploid F2 progeny. Fish were infected with F. psychrophilum either by intramuscular injection (115 individuals) or by immersion (195 individuals), and genotyped for 9654 markers using RAD-sequencing. Fifteen QTL associated with resistance traits were detected and only three QTL were common between the injection and immersion. Using a model that accounted for epistatic interactions between QTL, two main types of interactions were revealed. A "compensation-like" effect was detected between several pairs of QTL for the two modes of infection. An "enhancing-like" interaction effect was detected between four pairs of QTL. Integration of the QTL results with results of a previous transcriptomic analysis of response to F. psychrophilum infection resulted in a list of potential candidate immune genes that belong to four relevant functional categories (bacterial sensors, effectors of antibacterial immunity, inflammatory factors and interferon-stimulated genes). CONCLUSIONS: These results provide new insights into the genetic determinism of rainbow trout resistance to F. psychrophilum and confirm that some QTL with large effects are involved in this trait. For the first time, the role of epistatic interactions between resistance-associated QTL was evidenced. We found that the infection protocol used had an effect on the modulation of defence mechanisms and also identified relevant immune functional candidate genes.
dc.identifier.citation	Genetics Selection Evolution. 2018 Nov 16;50(1):60
dc.identifier.doi	10.17863/CAM.32762
dc.identifier.eissn	1297-9686
dc.identifier.issn	0999-193X
dc.identifier.uri	https://www.repository.cam.ac.uk/handle/1810/285396
dc.language.iso	eng
dc.language.rfc3066	en
dc.publisher	Springer Science and Business Media LLC
dc.publisher.url	http://dx.doi.org/10.1186/s12711-018-0431-9
dc.rights.holder	The Author(s)
dc.subject	Animals
dc.subject	Disease Resistance
dc.subject	Female
dc.subject	Fish Diseases
dc.subject	Flavobacteriaceae Infections
dc.subject	Flavobacterium
dc.subject	Genotype
dc.subject	Male
dc.subject	Oncorhynchus mykiss
dc.subject	Phenotype
dc.subject	Polymorphism, Single Nucleotide
dc.subject	Quantitative Trait Loci
dc.title	Quantitative trait loci for resistance to Flavobacterium psychrophilum in rainbow trout: effect of the mode of infection and evidence of epistatic interactions.
dc.type	Journal Article
dcterms.dateAccepted	2018-11-06
prism.publicationName	Genet Sel Evol
rioxxterms.versionofrecord	10.1186/s12711-018-0431-9

Quantitative trait loci for resistance to Flavobacterium psychrophilum in rainbow trout: effect of the mode of infection and evidence of epistatic interactions.

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