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Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Kauts, Mari-Liis 
De Leo, Bianca 
Rodríguez-Seoane, Carmen 
Ronn, Roger 
Glykofrydis, Fokion 

Abstract

Mast cells are tissue-resident immune cells. Their overgrowth/overactivation results in a range of common distressing, sometimes life-threatening disorders, including asthma, psoriasis, anaphylaxis, and mastocytosis. Currently, drug discovery is hampered by use of cancer-derived mast cell lines or primary cells. Cell lines provide low numbers of mature mast cells and are not representative of in vivo mast cells. Mast cell generation from blood/bone marrow gives poor reproducibility, requiring 8-12 weeks of culture. Here we report a method for the rapid/robust production of mast cells from pluripotent stem cells (PSCs). An advantageous Gata2Venus reporter enriches mast cells and progenitors as they differentiate from PSCs. Highly proliferative mouse mast cells and progenitors emerge after 2 weeks. This method is applicable for rapid human mast cell generation, and could enable the production of sufficient numbers of physiologically relevant human mast cells from patient induced PSCs for the study of mast cell-associated disorders and drug discovery.

Description

Keywords

ESC, Gata2, Venus reporter, differentiation, iPSC, immune effectors, innate immune cells, mast cells, rapid protocol, stem cells, Animals, Cell Culture Techniques, Cell Differentiation, Cells, Cultured, GATA2 Transcription Factor, Genes, Reporter, Humans, Mast Cells, Mice, Mouse Embryonic Stem Cells, Peptide Hydrolases, Phenotype, Pluripotent Stem Cells, Receptors, Cell Surface

Journal Title

Stem Cell Reports

Conference Name

Journal ISSN

2213-6711
2213-6711

Volume Title

11

Publisher

Elsevier BV
Sponsorship
Leukaemia & Lymphoma Research (12029)
Medical Research Council (MC_PC_12009)
CCF (None)