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Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells.

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cam.issuedOnline2018-09-06
dc.contributor.authorKauts, Mari-Liis
dc.contributor.authorDe Leo, Bianca
dc.contributor.authorRodríguez-Seoane, Carmen
dc.contributor.authorRonn, Roger
dc.contributor.authorGlykofrydis, Fokion
dc.contributor.authorMaglitto, Antonio
dc.contributor.authorKaimakis, Polynikis
dc.contributor.authorBasi, Margarita
dc.contributor.authorTaylor, Helen
dc.contributor.authorForrester, Lesley
dc.contributor.authorWilkinson, Adam C
dc.contributor.authorGöttgens, Berthold
dc.contributor.authorSaunders, Philippa
dc.contributor.authorDzierzak, Elaine
dc.contributor.orcidGottgens, Berthold [0000-0001-6302-5705]
dc.date.accessioned2018-11-17T00:31:40Z
dc.date.available2018-11-17T00:31:40Z
dc.date.issued2018-10-09
dc.description.abstractMast cells are tissue-resident immune cells. Their overgrowth/overactivation results in a range of common distressing, sometimes life-threatening disorders, including asthma, psoriasis, anaphylaxis, and mastocytosis. Currently, drug discovery is hampered by use of cancer-derived mast cell lines or primary cells. Cell lines provide low numbers of mature mast cells and are not representative of in vivo mast cells. Mast cell generation from blood/bone marrow gives poor reproducibility, requiring 8-12 weeks of culture. Here we report a method for the rapid/robust production of mast cells from pluripotent stem cells (PSCs). An advantageous Gata2Venus reporter enriches mast cells and progenitors as they differentiate from PSCs. Highly proliferative mouse mast cells and progenitors emerge after 2 weeks. This method is applicable for rapid human mast cell generation, and could enable the production of sufficient numbers of physiologically relevant human mast cells from patient induced PSCs for the study of mast cell-associated disorders and drug discovery.
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.32742
dc.identifier.eissn2213-6711
dc.identifier.issn2213-6711
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285376
dc.languageeng
dc.language.isoeng
dc.publisherElsevier BV
dc.publisher.urlhttp://dx.doi.org/10.1016/j.stemcr.2018.08.007
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectESC
dc.subjectGata2
dc.subjectVenus reporter
dc.subjectdifferentiation
dc.subjectiPSC
dc.subjectimmune effectors
dc.subjectinnate immune cells
dc.subjectmast cells
dc.subjectrapid protocol
dc.subjectstem cells
dc.subjectAnimals
dc.subjectCell Culture Techniques
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectGATA2 Transcription Factor
dc.subjectGenes, Reporter
dc.subjectHumans
dc.subjectMast Cells
dc.subjectMice
dc.subjectMouse Embryonic Stem Cells
dc.subjectPeptide Hydrolases
dc.subjectPhenotype
dc.subjectPluripotent Stem Cells
dc.subjectReceptors, Cell Surface
dc.titleRapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells.
dc.typeArticle
dcterms.dateAccepted2018-08-08
prism.endingPage1020
prism.issueIdentifier4
prism.publicationDate2018
prism.publicationNameStem Cell Reports
prism.startingPage1009
prism.volume11
pubs.funder-project-idLeukaemia & Lymphoma Research (12029)
pubs.funder-project-idMedical Research Council (MC_PC_12009)
pubs.funder-project-idCCF (None)
rioxxterms.licenseref.startdate2018-10
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1016/j.stemcr.2018.08.007

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