Repository logo
 

Centrosome docking at the immunological synapse is controlled by Lck signaling.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Tsun, Andy 
Qureshi, Ihjaaz 
Stinchcombe, Jane C 
Jenkins, Misty R 

Abstract

Docking of the centrosome at the plasma membrane directs lytic granules to the immunological synapse. To identify signals controlling centrosome docking at the synapse, we have studied cytotoxic T lymphocytes (CTLs) in which expression of the T cell receptor-activated tyrosine kinase Lck is ablated. In the absence of Lck, the centrosome is able to translocate around the nucleus toward the immunological synapse but is unable to dock at the plasma membrane. Lytic granules fail to polarize and release their contents, and target cells are not killed. In CTLs deficient in both Lck and the related tyrosine kinase Fyn, centrosome translocation is impaired, and the centrosome remains on the distal side of the nucleus relative to the synapse. These results show that repositioning of the centrosome in CTLs involves at least two distinct steps, with Lck signaling required for the centrosome to dock at the plasma membrane.

Description

Keywords

Animals, Cell Membrane, Cell Nucleus, Centrosome, Cytoplasmic Granules, Immunological Synapses, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Mice, Mice, Transgenic, Proto-Oncogene Proteins c-fyn, Receptors, Antigen, T-Cell, Signal Transduction, T-Lymphocytes, Cytotoxic

Journal Title

J Cell Biol

Conference Name

Journal ISSN

0021-9525
1540-8140

Volume Title

192

Publisher

Rockefeller University Press
Sponsorship
Wellcome Trust (75880)