Assessing the Collective Dynamics of Motile Cilia in Cultures of Human Airway Cells by Multiscale DDM
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Peer-reviewed
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Abstract
The technique of differential dynamic microscopy is extended here, showing that it can provide a powerful and objective method of video analysis for optical microscopy videos of in vitro samples of live human bronchial epithelial ciliated cells. These cells are multiciliated, with motile cilia that play key physiological roles. It is shown that the ciliary beat frequency can be recovered to match conventional analysis, but in a fully automated fashion. Furthermore, it is shown that the properties of spatial and temporal coherence of cilia beat can be recovered and distinguished, and that if a collective traveling wave (the metachronal wave) is present, this has a distinct signature and its wavelength and direction can be measured.
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Keywords
Automation, Laboratory, Bronchi, Cells, Cultured, Cilia, Epithelial Cells, Humans, Microscopy, Video, Nasal Mucosa, Optical Imaging
Journal Title
Biophysical Journal
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Journal ISSN
0006-3495
1542-0086
1542-0086
Volume Title
113
Publisher
Elsevier
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Sponsorship
Biotechnology and Biological Sciences Research Council (BB/H021930/1)
Wellcome Trust (108045/Z/15/Z)
Wellcome Trust (108045/Z/15/Z)
L.F., M.C., and P.C. are funded by the EU-ERC CoG HydroSync grant.