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Type 3 Secretion System (T3SS) of Bradyrhizobium sp. DOA9 and Its Roles in Legume Symbiosis and Rice Endophytic Association.

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cam.depositDate2021-12-06
cam.issuedOnline2017-09-20
dc.contributor.authorSongwattana, Pongpan
dc.contributor.authorNoisangiam, Rujirek
dc.contributor.authorTeamtisong, Kamonluck
dc.contributor.authorPrakamhang, Janpen
dc.contributor.authorTeulet, Albin
dc.contributor.authorTittabutr, Panlada
dc.contributor.authorPiromyou, Pongdet
dc.contributor.authorBoonkerd, Nantakorn
dc.contributor.authorGiraud, Eric
dc.contributor.authorTeaumroong, Neung
dc.contributor.orcidTeulet, Albin [0000-0002-3188-7260]
dc.date.accessioned2021-12-15T00:30:20Z
dc.date.available2021-12-15T00:30:20Z
dc.date.issued2017
dc.date.updated2021-12-06T21:36:13Z
dc.description.abstractThe Bradyrhizobium sp. DOA9 strain isolated from a paddy field has the ability to nodulate a wide spectrum of legumes. Unlike other bradyrhizobia, this strain has a symbiotic plasmid harboring nod, nif, and type 3 secretion system (T3SS) genes. This T3SS cluster contains all the genes necessary for the formation of the secretory apparatus and the transcriptional activator (TtsI), which is preceded by a nod-box motif. An in silico search predicted 14 effectors putatively translocated by this T3SS machinery. In this study, we explored the role of the T3SS in the symbiotic performance of DOA9 by evaluating the ability of a T3SS mutant (ΩrhcN) to nodulate legumes belonging to Dalbergioid, Millettioid, and Genistoid tribes. Among the nine species tested, four (Arachis hypogea, Vigna radiata, Crotalaria juncea, and Macroptilium atropurpureum) responded positively to the rhcN mutation (ranging from suppression of plant defense reactions, an increase in the number of nodules and a dramatic improvement in nodule development and infection), one (Stylosanthes hamata) responded negatively (fewer nodules and less nitrogen fixation) and four species (Aeschynomene americana, Aeschynomene afraspera, Indigofera tinctoria, and Desmodium tortuosum) displayed no phenotype. We also tested the role of the T3SS in the ability of the DOA9 strain to endophytically colonize rice roots, but detected no effect of the T3SS mutation, in contrast to what was previously reported in the Bradyrhizobium SUTN9-2 strain. Taken together, these data indicate that DOA9 contains a functional T3SS that interferes with the ability of the strain to interact symbiotically with legumes but not with rice.
dc.identifier.doi10.17863/CAM.78845
dc.identifier.eissn1664-302X
dc.identifier.issn1664-302X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331392
dc.language.isoeng
dc.publisherFrontiers Media SA
dc.publisher.departmentSainsbury Laboratory
dc.publisher.urlhttp://dx.doi.org/10.3389/fmicb.2017.01810
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBradyrhizobium
dc.subjecteffector proteins
dc.subjectlegume
dc.subjectsymbiosis
dc.subjecttype 3 secretion system
dc.titleType 3 Secretion System (T3SS) of Bradyrhizobium sp. DOA9 and Its Roles in Legume Symbiosis and Rice Endophytic Association.
dc.typeArticle
dcterms.dateAccepted2017-09-05
prism.publicationDate2017
prism.publicationNameFront Microbiol
prism.startingPage1810
prism.volume8
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
pubs.licence-identifierapollo-deposit-licence-2-1
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.3389/fmicb.2017.01810

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