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Mouse Primordial Germ Cell-Like Cells Lack piRNAs

Published version
Peer-reviewed

Type

Article

Change log

Authors

Ramakrishna, Navin B 
Battistoni, Giorgia 
Surani, Azim 
Hannon, Gregory 

Abstract

PIWI-interacting RNAs (piRNAs) are small RNAs bound by PIWI-clade Argonaute proteins that function to silence transposable elements (TEs). Following mouse Primordial Germ Cell (mPGC) specification around E6.25, fetal piRNAs subsequently emerge in male gonocytes from E13.5 onwards. The in vitro differentiation of mPGC-Like Cells (mPGCLCs) from mESCs has raised the tantalizing prospect of studying the fetal piRNA pathway in greater depth. However, using single-cell RNA-seq and RT-qPCR along mPGCLC differentiation, we find that piRNA pathway factors are not yet expressed in D6 mPGCLCs. Moreover, we do not detect piRNAs across a panel of D6 mPGCLC lines using small RNA-seq. Our combined efforts from two laboratories highlight that in vitro differentiated D6 mPGCLCs do not yet resemble E13.5 or later mouse gonocytes where the piRNA pathway is active, in contrast to a prior report.

Description

Keywords

PIWI, mPGC, mPGCLC, piRNA, primordial germ cell, Male, Mice, Animals, Piwi-Interacting RNA, Germ Cells

Journal Title

Developmental Cell

Conference Name

Journal ISSN

1534-5807
1878-1551

Volume Title

Publisher

Elsevier
Sponsorship
Wellcome Trust (203144/A/16/Z)
Wellcome Trust (203144/Z/16/Z)
Cancer Research UK (C6946/A24843)
Wellcome Trust (219475/Z/19/Z)
Wellcome Trust (092096/Z/10/Z)
Wellcome Trust (209475/Z/17/Z)
Wellcome Trust (110161/Z/15/Z)
Royal Society (RP130039)
Royal Society (RSRP\R\200001)
Cancer Research Uk (None)
Cancer Research UK (18583)