Correct regionalization of a tissue primordium is essential for coordinated morphogenesis.

Sánchez-Corrales, Yara E; Blanchard, Guy; Röper, Katja

Correct regionalization of a tissue primordium is essential for coordinated morphogenesis.

cam.issuedOnline	2021-11-01
datacite.contributor.supervisor	editor: Devenport, Danelle
datacite.contributor.supervisor	senior_editor: Akhmanova, Anna
dc.contributor.author	Sánchez-Corrales, Yara E
dc.contributor.author	Blanchard, Guy
dc.contributor.author	Röper, Katja
dc.contributor.orcid	Sánchez-Corrales, Yara E [0000-0003-1438-1994]
dc.contributor.orcid	Blanchard, Guy [0000-0002-3689-0522]
dc.contributor.orcid	Röper, Katja [0000-0002-3361-766X]
dc.date.accessioned	2021-11-22T14:44:01Z
dc.date.available	2021-11-22T14:44:01Z
dc.date.issued	2021-11-01
dc.date.submitted	2021-07-21
dc.date.updated	2021-11-22T14:43:56Z
dc.description.abstract	During organ development, tubular organs often form from flat epithelial primordia. In the placodes of the forming tubes of the salivary glands in the Drosophila embryo, we previously identified spatially defined cell behaviors of cell wedging, tilting, and cell intercalation that are key to the initial stages of tube formation. Here, we address what the requirements are that ensure the continuous formation of a narrow symmetrical tube from an initially asymmetrical primordium whilst overall tissue geometry is constantly changing. We are using live-imaging and quantitative methods to compare wild-type placodes and mutants that either show disrupted cell behaviors or an initial symmetrical placode organization, with both resulting in severe impairment of the invagination. We find that early transcriptional patterning of key morphogenetic transcription factors drives the selective activation of downstream morphogenetic modules, such as GPCR signaling that activates apical-medial actomyosin activity to drive cell wedging at the future asymmetrically placed invagination point. Over time, transcription of key factors expands across the rest of the placode and cells switch their behavior from predominantly intercalating to predominantly apically constricting as their position approaches the invagination pit. Misplacement or enlargement of the initial invagination pit leads to early problems in cell behaviors that eventually result in a defective organ shape. Our work illustrates that the dynamic patterning of the expression of transcription factors and downstream morphogenetic effectors ensures positionally fixed areas of cell behavior with regards to the invagination point. This patterning in combination with the asymmetric geometrical setup ensures functional organ formation.
dc.identifier.doi	10.17863/CAM.78312
dc.identifier.eissn	2050-084X
dc.identifier.issn	2050-084X
dc.identifier.other	72369
dc.identifier.uri	https://www.repository.cam.ac.uk/handle/1810/330869
dc.language	en
dc.publisher	eLife Sciences Publications, Ltd
dc.subject	Research Article
dc.subject	Cell Biology
dc.subject	Developmental Biology
dc.subject	organogenesis
dc.subject	tubulogenesis
dc.subject	morphogenesis
dc.subject	morphometrics
dc.subject	apical constriction
dc.subject	intercalation
dc.subject	D. melanogaster
dc.title	Correct regionalization of a tissue primordium is essential for coordinated morphogenesis.
dc.type	Article
dcterms.dateAccepted	2021-10-31
prism.publicationName	Elife
prism.volume	10
pubs.funder-project-id	Wellcome Trust (207553/Z/17/Z)
rioxxterms.licenseref.uri	http://creativecommons.org/licenses/by/4.0/
rioxxterms.version	VoR
rioxxterms.versionofrecord	10.7554/eLife.72369