Repository logo
 

Daughter centrioles assemble preferentially towards the nuclear envelope in Drosophila syncytial embryos.

Simple item page
cam.issuedOnline2022-01-19
dc.contributor.authorCunningham, Neil HJ
dc.contributor.authorBouhlel, Imène B
dc.contributor.authorConduit, Paul T
dc.contributor.orcidBouhlel, Imène B [0000-0001-5876-4597]
dc.contributor.orcidConduit, Paul T [0000-0002-7822-1191]
dc.date.accessioned2022-02-20T02:02:34Z
dc.date.available2022-02-20T02:02:34Z
dc.date.issued2022-01
dc.date.updated2022-02-20T02:02:33Z
dc.description.abstractCentrosomes are important organizers of microtubules within animal cells. They comprise a pair of centrioles surrounded by the pericentriolar material, which nucleates and organizes the microtubules. To maintain centrosome numbers, centrioles must duplicate once and only once per cell cycle. During S-phase, a single new 'daughter' centriole is built orthogonally on one side of each radially symmetric 'mother' centriole. Mis-regulation of duplication can result in the simultaneous formation of multiple daughter centrioles around a single mother centriole, leading to centrosome amplification, a hallmark of cancer. It remains unclear how a single duplication site is established. It also remains unknown whether this site is pre-defined or randomly positioned around the mother centriole. Here, we show that within Drosophila syncytial embryos daughter centrioles preferentially assemble on the side of the mother facing the nuclear envelope, to which the centrosomes are closely attached. This positional preference is established early during duplication and remains stable throughout daughter centriole assembly, but is lost in centrosomes forced to lose their connection to the nuclear envelope. This shows that non-centrosomal cues influence centriole duplication and raises the possibility that these external cues could help establish a single duplication site.
dc.identifier.doi10.17863/CAM.81653
dc.identifier.eissn2046-2441
dc.identifier.issn2046-2441
dc.identifier.otherPMC8767211
dc.identifier.other35042404
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/334240
dc.languageeng
dc.publisherThe Royal Society
dc.publisher.urlhttp://dx.doi.org/10.1098/rsob.210343
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcenlmid: 101580419
dc.sourceessn: 2046-2441
dc.subjectDrosophila
dc.subjectcentriole
dc.subjectcentriole duplication
dc.subjectcentrosome
dc.subjectmicrotubules
dc.subjectAnimals
dc.subjectCentrioles
dc.subjectCentrosome
dc.subjectDrosophila
dc.subjectDrosophila Proteins
dc.subjectNuclear Envelope
dc.titleDaughter centrioles assemble preferentially towards the nuclear envelope in Drosophila syncytial embryos.
dc.typeArticle
dcterms.dateAccepted2021-12-16
prism.issueIdentifier1
prism.publicationNameOpen Biol
prism.volume12
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/P019188/1)
pubs.funder-project-idWellcome Trust (105653/Z/14/Z)
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1098/rsob.210343

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
article.pdf
Size:
1.4 MB
Format:
Adobe Portable Document Format
Description:
Published version
Licence
https://creativecommons.org/licenses/by/4.0/
Download

Collections

Jisc Publications Router