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Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Frangedakis, Eftychios  ORCID logo  https://orcid.org/0000-0002-3483-8464
Guzman-Chavez, Fernando 
Yu, Ying 

Abstract

Chloroplasts are attractive platforms for synthetic biology applications since they are capable of driving very high levels of transgene expression, if mRNA production and stability are properly regulated. However, plastid transformation is a slow process and currently limited to a few plant species. The liverwort Marchantia polymorpha is a simple model plant that allows rapid transformation studies; however, its potential for protein hyperexpression has not been fully exploited. This is partially due to the fact that chloroplast post-transcriptional regulation is poorly characterized in this plant. We have mapped patterns of transcription in Marchantia chloroplasts. Furthermore, we have obtained and compared sequences from 51 bryophyte species and identified putative sites for pentatricopeptide repeat protein binding that are thought to play important roles in mRNA stabilization. Candidate binding sites were tested for their ability to confer high levels of reporter gene expression in Marchantia chloroplasts, and levels of protein production and effects on growth were measured in homoplastic transformed plants. We have produced novel DNA tools for protein hyperexpression in this facile plant system that is a test-bed for chloroplast engineering.

Description

Keywords

Marchantia, chloroplast, gene assembly, plant, plastome, transcriptome, Chloroplasts, DNA, Recombinant, Genes, Plant, Marchantia, Plant Proteins, Protein Binding, Synthetic Biology, Transcription, Genetic, Transcriptome, Transformation, Genetic

Journal Title

ACS Synth Biol

Conference Name

Journal ISSN

2161-5063
2161-5063

Volume Title

10

Publisher

American Chemical Society (ACS)
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/L014130/1)
Biotechnology and Biological Sciences Research Council (BB/F011458/1)
BBSRC (BB/T007117/1)
BBSRC (1943399)