Repository logo
 

The calcium transporter ANNEXIN1 mediates cold-induced calcium signaling and freezing tolerance in plants.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Liu, Qiangbo 
Ding, Yanglin 
Shi, Yiting 
Ma, Liang 

Abstract

The transient elevation of cytosolic free calcium concentration ([Ca2+ ]cyt ) induced by cold stress is a well-established phenomenon; however, the underlying mechanism remains elusive. Here, we report that the Ca2+ -permeable transporter ANNEXIN1 (AtANN1) mediates cold-triggered Ca2+ influx and freezing tolerance in Arabidopsis thaliana. The loss of function of AtANN1 substantially impaired freezing tolerance, reducing the cold-induced [Ca2+ ]cyt increase and upregulation of the cold-responsive CBF and COR genes. Further analysis showed that the OST1/SnRK2.6 kinase interacted with and phosphorylated AtANN1, which consequently enhanced its Ca2+ transport activity, thereby potentiating Ca2+ signaling. Consistent with these results and freezing sensitivity of ost1 mutants, the cold-induced [Ca2+ ]cyt elevation in the ost1-3 mutant was reduced. Genetic analysis indicated that AtANN1 acts downstream of OST1 in responses to cold stress. Our data thus uncover a cascade linking OST1-AtANN1 to cold-induced Ca2+ signal generation, which activates the cold response and consequently enhances freezing tolerance in Arabidopsis.

Description

Keywords

Arabidopsis, OST1 kinase, calcium signal, calcium-permeable transporter AtANN1, freezing tolerance, Arabidopsis, Arabidopsis Proteins, Calcium, Calcium Signaling, Cell Membrane, Cold Temperature, Cold-Shock Response, Freezing, Gene Expression Regulation, Plant, Protein Kinases, Transcription Factors

Journal Title

EMBO J

Conference Name

Journal ISSN

0261-4189
1460-2075

Volume Title

40

Publisher

Springer Science and Business Media LLC

Rights

All rights reserved
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/K009869/1)