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The architecture of Cidec-mediated interfaces between lipid droplets.

Accepted version
Peer-reviewed

Type

Article

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Authors

Ganeva, Iva 
Lim, Koini 
Boulanger, Jerome 
Hoffmann, Patrick C 
Muriel, Olivia 

Abstract

Lipid droplets (LDs) are intracellular organelles responsible for storing surplus energy as neutral lipids. Their size and number vary enormously. In white adipocytes, LDs can reach 100 μm in diameter, occupying >90% of the cell. Cidec, which is strictly required for the formation of large LDs, is concentrated at interfaces between adjacent LDs and facilitates directional flux of neutral lipids from the smaller to the larger LD. The mechanism of lipid transfer is unclear, in part because the architecture of interfaces between LDs remains elusive. Here we visualize interfaces between LDs by electron cryo-tomography and analyze the kinetics of lipid transfer by quantitative live fluorescence microscopy. We show that transfer occurs through closely apposed monolayers, is slowed down by increasing the distance between the monolayers, and follows exponential kinetics. Our data corroborate the notion that Cidec facilitates pressure-driven transfer of neutral lipids through two "leaky" monolayers between LDs.

Description

Keywords

CP: Cell biology, Cidec, cryo-CLEM, cryo-ET, lipid droplets, lipid transfer, Lipid Droplets, Proteins, Lipids, Lipid Metabolism

Journal Title

Cell Rep

Conference Name

Journal ISSN

2211-1247
2211-1247

Volume Title

Publisher

Elsevier BV
Sponsorship
Wellcome Trust (219417/Z/19/Z)
Medical Research Council (MC_UU_12012/1)
Wellcome Trust (208363/Z/17/Z)
Medical Research Council (MC_PC_12012)