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Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis.

Published version
Peer-reviewed

Type

Article

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Authors

Abstract

The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphisms in SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), which encodes a component of the SMC5/6 complex. The sni1 mutant exhibits a modified pattern of recombination across the genome with crossovers elevated in chromosome distal regions but reduced in pericentromeres. Mutations in SNI1 result in reduced crossover interference and can partially restore the fertility of a Class I crossover pathway mutant, which suggests that the protein affects noninterfering crossover repair. Therefore, we tested genetic interactions between SNI1 and both RECQ4 and FANCM DNA helicases, which showed that additional Class II crossovers observed in the sni1 mutant are FANCM independent. Furthermore, genetic analysis of other SMC5/6 mutants confirms the observations of crossover redistribution made for SNI1 The study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants.

Description

Keywords

Arabidopsis, SMC5/6, SNI1, crossover, meiosis, Arabidopsis, Arabidopsis Proteins, Crossing Over, Genetic, DNA Helicases, Gene Expression Regulation, Plant, Genetic Variation, Meiosis, Nuclear Proteins, Protein Domains

Journal Title

Proc Natl Acad Sci U S A

Conference Name

Journal ISSN

0027-8424
1091-6490

Volume Title

118

Publisher

Proceedings of the National Academy of Sciences
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/L006847/1)
Biotechnology and Biological Sciences Research Council (BB/M004937/1)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (765212)
Biotechnology and Biological Sciences Research Council (BB/S006842/1)
BBSRC (BB/T004282/1)
BBSRC (BB/V003984/1)