G-quadruplex structures mark human regulatory chromatin
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G-quadruplex (G4) structural motifs have been linked to transcription, replication and genome instability and are implicated in cancer and other diseases. However, it is crucial to demonstrate the bona fide formation of G4 structures within an endogenous chromatin context. Herein we address this through the development of G4 ChIP-seq, an antibody-based G4 chromatin immunoprecipitation and high-throughput sequencing approach. We find ∼10,000 G4 structures in human chromatin, predominantly in regulatory, nucleosome-depleted regions. G4 structures are enriched in the promoters and 5' UTRs of highly transcribed genes, particularly in genes related to cancer and in somatic copy number amplifications, such as
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1546-1718
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Wellcome Trust (099232/Z/12/Z)
Cancer Research UK (CB4330)
Cancer Research UK (18618)
Cancer Research UK (19836)
Cancer Research UK (15890)