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Genome-wide assessment of the carriers involved in the cellular uptake of drugs: a model system in yeast.

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Lanthaler, Karin 
Bilsland, Elizabeth 
Dobson, Paul D 
Moss, Harry J 
Pir, Pınar 


BACKGROUND: The uptake of drugs into cells has traditionally been considered to be predominantly via passive diffusion through the bilayer portion of the cell membrane. The recent recognition that drug uptake is mostly carrier-mediated raises the question of which drugs use which carriers. RESULTS: To answer this, we have constructed a chemical genomics platform built upon the yeast gene deletion collection, using competition experiments in batch fermenters and robotic automation of cytotoxicity screens, including protection by 'natural' substrates. Using these, we tested 26 different drugs and identified the carriers required for 18 of the drugs to gain entry into yeast cells. CONCLUSIONS: As well as providing a useful platform technology, these results further substantiate the notion that the cellular uptake of pharmaceutical drugs normally occurs via carrier-mediated transport and indicates that establishing the identity and tissue distribution of such carriers should be a major consideration in the design of safe and effective drugs.



Biological Transport, Canavanine, Cell Membrane, Cell Membrane Permeability, Drug Evaluation, Preclinical, Gene Deletion, Genome-Wide Association Study, Genomics, Humans, Membrane Transport Proteins, Pharmaceutical Preparations, Polymerase Chain Reaction, Saccharomyces cerevisiae

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BMC Biol

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Springer Science and Business Media LLC
Medical Research Council (MC_G1000734)
Biotechnology and Biological Sciences Research Council (BB/F008228/1)