Repository logo

Application of single-cell RNA sequencing methodologies in understanding haematopoiesis and immunology.

Published version

Change log


Ranzoni, Anna M 
Strzelecka, Paulina M 


The blood and immune system are characterised by utmost diversity in its cellular components. This heterogeneity can solely be resolved with the application of single-cell technologies that enable precise examination of cell-to-cell variation. Single-cell transcriptomics is continuously pushing forward our understanding of processes driving haematopoiesis and immune responses in physiological settings as well as in disease. Remarkably, in the last five years, a number of studies involving single-cell RNA sequencing (scRNA-seq) allowed the discovery of new immune cell types and revealed that haematopoiesis is a continuous rather than a stepwise process, thus challenging the classical haematopoietic lineage tree model. This review summarises the most recent studies which applied scRNA-seq to answer outstanding questions in the fields of haematology and immunology and discusses the present challenges and future directions.



Single-cell RNA sequencing, haematopoiesis, immunology, Animals, Cells, Cultured, Hematopoiesis, Humans, Immune System, Mice, RNA, Small Cytoplasmic, Sequence Analysis, RNA, Single-Cell Analysis

Journal Title

Essays Biochem

Conference Name

Journal ISSN


Volume Title



Portland Press Ltd.
Cancer Research Uk (None)
European Research Council (677501)
Medical Research Council (MC_PC_12009)
The study was supported by Cancer Research UK grant number C45041/A14953 and European Research Council project 677501 – ZF_Blood