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Tailoring chitosan/collagen scaffolds for tissue engineering: Effect of composition and different crosslinking agents on scaffold properties.

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Martínez, A 
Blanco, MD 
Davidenko, N 
Cameron, RE 


Chitosan/collagen (Chit/Col) blends have demonstrated great potential for use in tissue engineering (TE) applications. However, there exists a lack of detailed study on the influence of important design parameters (i.e, component ratio or crosslinking methods) on the essential properties of the scaffolds (morphology, mechanical stiffness, swelling, degradation and cytotoxicity). This work entailed a systematic study of these essential properties of three Chit/Col compositions, covering a wide range of component ratios and using different crosslinking methods. Our results showed the possibility of tailoring these properties by changing component ratios, since different interactions occurred between Chit/Col: samples with Chit-enriched compositions showed a hydrogen-bonding type complex (HC), whereas a self-crosslinking phenomenon was induced in Col-enriched scaffolds. Additionally, material and biological properties of the resultant matrices were further adjusted and tuned by changing crosslinking conditions. In such way, we obtained a wide range of scaffolds whose properties were tailored to meet specific needs of TE applications.



Chitosan, Collagen, Crosslinking (XL), Scaffolds, Tissue engineering (TE), Biocompatible Materials, Cell Culture Techniques, Cell Proliferation, Chitosan, Collagen, Collagenases, Humans, MCF-7 Cells, Materials Testing, Muramidase, Porosity, Tissue Engineering, Tissue Scaffolds

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Elsevier BV
The authors are grateful to Dr. von Kobbe (Chimera Pharma of Bionostra Group) for the gift of MCF7 cells. The financial support of the Ministerio de Ciencia e Innovación of Spain (FIS PS09/01513), and the FPI grant from UCM to A. Martínez are gratefully acknowledged.