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Zein as biodegradable material for effective delivery of alkaline phosphatase and substrates in biokits and biosensors

Accepted version
Peer-reviewed

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Type

Article

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Authors

Jornet-Martínez, N 
Campíns-Falcó, P 
Hall, EAH 

Abstract

A biodegradable material, zein, is proposed as a reagent delivery platform for biokits and biosensors based on alkaline phosphatase (ALP) activity/inhibition in the presence of phosphatase substrates. The immobilization and release of both the substrate and/or the active ALP, in a biodegradable and low-cost material such as zein, a prolamin from maize, and in combination with glycerol as plasticizer have been investigated. Three zein-based devices are proposed for several applications: (1) inorganic phosphorus estimation in water of different sources (river, lake, coastal water and tap water) with a detection limit of 0.2mg/L - compared to at least 1mg/L required by legislation, (2) estimation of ALP in saliva and (3) chlorpyrifos control in commercial preparations. The single-use kits developed are low cost, easy and fast to manufacture and are stable for at least 20 days at -20°C, so the zein film can preserve and deliver both the enzyme and substrates.

Description

Keywords

Alkaline phosphatase, Biomaterial, Biosensors, Pesticide, Saliva, Zein

Journal Title

Biosensors and Bioelectronics

Conference Name

Journal ISSN

0956-5663
1873-4235

Volume Title

86

Publisher

Elsevier
Sponsorship
The authors are grateful to Generalitat Valenciana (PROMETEO 2012/045 project and BEFPI/2014/014), Spain and to the Spanish Ministerio de Economía y Competitividad/FEDER (Project CTQ2014-53916-P). NJ expresses her gratitude to PROMETEO program for her predoctoral grant and BEFPI program for her stay grant.