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The process of EDC-NHS Cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment.


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Type

Article

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Authors

Shepherd, DV 
Shepherd, JH 
Ghose, S 
Kew, SJ 
Cameron, RE 

Abstract

We describe the production of collagen fibre bundles through a multi-strand, semi-continuous extrusion process. Cross-linking using an EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide), NHS (N-hydroxysuccinimide) combination was considered. Atomic Force Microscopy (AFM) and Raman spectroscopy focused on how cross-linking affected the collagen fibrillar structure. In the cross-linked fibres, a clear fibrillar structure comparable to native collagen was observed which was not observed in the non-cross-linked fibre. The amide III doublet in the Raman spectra provided additional evidence of alignment in the cross-linked fibres. Raman spectroscopy also indicated no residual polyethylene glycol (from the fibre forming buffer) or water in any of the fibres.

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Keywords

0306 Physical Chemistry (incl. Structural)

Journal Title

APL Mater

Conference Name

Journal ISSN

2166-532X
2166-532X

Volume Title

3

Publisher

AIP Publishing
Sponsorship
Engineering and Physical Sciences Research Council (DT/F006977/1)
The authors would like to acknowledge the support of the Engineering and Physical Sciences Research Council (EPSRC), UK through a Knowledge Transfer Secondment (KTS) (to JHS), The National Institute for Health Research (NIHR) through their i4i grant to Tigenix Ltd and the TSB grant TP/8/BIO/6/I/Q0052.