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Cations Regulate Membrane Attachment and Functionality of DNA Nanostructures.

Accepted version
Peer-reviewed

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Article

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Abstract

The interplay between nucleic acids and lipids underpins several key processes in molecular biology, synthetic biotechnology, vaccine technology, and nanomedicine. These interactions are often electrostatic in nature, and much of their rich phenomenology remains unexplored in view of the chemical diversity of lipids, the heterogeneity of their phases, and the broad range of relevant solvent conditions. Here we unravel the electrostatic interactions between zwitterionic lipid membranes and DNA nanostructures in the presence of physiologically relevant cations, with the purpose of identifying new routes to program DNA-lipid complexation and membrane-active nanodevices. We demonstrate that this interplay is influenced by both the phase of the lipid membranes and the valency of the ions and observe divalent cation bridging between nucleic acids and gel-phase bilayers. Furthermore, even in the presence of hydrophobic modifications on the DNA, we find that cations are still required to enable DNA adhesion to liquid-phase membranes. We show that the latter mechanism can be exploited to control the degree of attachment of cholesterol-modified DNA nanostructures by modifying their overall hydrophobicity and charge. Besides their biological relevance, the interaction mechanisms we explored hold great practical potential in the design of biomimetic nanodevices, as we show by constructing an ion-regulated DNA-based synthetic enzyme.

Description

Keywords

Cations, DNA, Lipid Bilayers, Molecular Dynamics Simulation, Nanostructures, Static Electricity

Journal Title

J Am Chem Soc

Conference Name

Journal ISSN

0002-7863
1520-5126

Volume Title

143

Publisher

American Chemical Society (ACS)

Rights

All rights reserved
Sponsorship
European Research Council (647144)
EPSRC (1948702)
Engineering and Physical Sciences Research Council (EP/L015978/1)
Royal Society (UF160152)
EPSRC (1949809)
Winton Programme for the Physics of Sustainability Engineering and Physical Sciences Research Council (NanoDTC, Grant No. EP/L015978/1 and Studentship project ref. 1948702). Mexican National Council for Science and Technology (CONACYT, Grant No. 472427) Cambridge Trust Royal Society University Research Fellowship (UF160152) European Research Council (ERC-STG No 851667 NANOCELL and DesignerPores 647144) National Science Foundation USA (DMR-1827346) Human Frontier Science Project (RGP0047/2020) XSEDE allocation grant (MCA05S028) Leadership Resource Allocation (MCB20012)