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Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells.

Published version
Peer-reviewed

Type

Article

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Authors

Welby, Emily 
Lakowski, Jorn 
Di Foggia, Valentina 
Budinger, Dimitri 
Gonzalez-Cordero, Anai 

Abstract

Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9.pR2.1:GFP reporter. This led to the identification of a cone-enriched gene signature, which we used to demonstrate similar gene expression between fetal and stem cell-derived cones. We then defined a cluster of differentiation marker combination that, when used for cell sorting, significantly enriches for cone photoreceptors from the fetal retina and stem cell-derived retinal organoids, respectively. These data may facilitate more efficient isolation of human stem cell-derived cones for use in clinical transplantation studies.

Description

Keywords

cell surface markers, cell transplantation therapy, cone photoreceptor cells, human pluripotent stem cells, retinal dystrophies, retinal organoids, transcriptome, Cell Differentiation, Fetus, Gene Expression Profiling, Gene Expression Regulation, Developmental, Humans, Induced Pluripotent Stem Cells, Retina, Retinal Cone Photoreceptor Cells, Retinal Degeneration, Rod Opsins, Transcriptome

Journal Title

Stem Cell Reports

Conference Name

Journal ISSN

2213-6711
2213-6711

Volume Title

9

Publisher

Elsevier BV
Sponsorship
Cancer Research UK (22231)