A direct role for SNX9 in the biogenesis of filopodia

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Jarsch, Iris 
Nuccitelli, Annalisa 
Mason, Julia 
Shimo, Hanae 

Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.

Animals, Female, HeLa Cells, Humans, Male, Pseudopodia, Sorting Nexins, Xenopus Proteins, Xenopus laevis
Journal Title
The Journal of Cell Biology
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Journal ISSN
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Rockefeller University Press
European Research Council (281971)
Wellcome Trust (108467/Z/15/Z)
Wellcome Trust (092096/Z/10/Z)
Medical Research Council (MR/N000846/1)
Cancer Research Uk (None)
Wellcome Trust (105602/Z/14/Z)
MRC (MR/T030089/1)
Cancer Research UK (A17196) European Research Council (615258) Medical Research Council (MC_UU_12016/2) Wellcome Trust (WT095829AIA)
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