Structural Analysis using SHALiPE to Reveal RNA GâQuadruplex Formation in Human Precursor MicroRNA
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jats:titleAbstract</jats:title>jats:pRNA Gâquadruplex (rG4) structures are of fundamental importance to biology. A novel approach is introduced to detect and structurally map rG4s at singleânucleotide resolution in RNAs. The approach, denoted SHALiPE, couples selective 2â˛âhydroxyl acylation with lithium ionâbased primer extension, and identifies characteristic structural fingerprints for rG4 mapping. We apply SHALiPE to interrogate the human precursor microRNAâ 149, and reveal the formation of an rG4 structure in this nonâcoding RNA. Additional analyses support the SHALiPE results and uncover that this rG4 has a parallel topology, is thermally stable, and is conserved in mammals. An inâ vitro Dicer assay shows that this rG4 inhibits Dicer processing, supporting the potential role of rG4 structures in microRNA maturation and postâtranscriptional regulation of mRNAs.</jats:p>
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1521-3757
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Cancer Research Uk (None)