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Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice

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Oshota, O 
Conway, M 
Fookes, M 
Schreiber, F 
Chaudhuri, R 


Salmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Consequently, in order to continue growing in the tissues, S. enterica requires the coordinated regulation of fitness genes. Bacterial gene regulation has so far been investigated largely using exposure to artificial environmental conditions or to in vitro cultured cells, and little information is available on how S. enterica adapts in vivo to sustain cell division and survival. We have studied the transcriptome, proteome and metabolic flux of Salmonella, and the transcriptome of the host during infection of wild type C57BL/6 and immune-deficient gp91-/-phox mice. Our analyses advance the understanding of how S. enterica and the host behaves during infection to a more sophisticated level than has previously been reported.



Animals, Bacterial Proteins, Female, Gene Deletion, Gene Expression Regulation, Bacterial, Genes, Bacterial, Immunologic Deficiency Syndromes, Male, Mice, Mice, Inbred C57BL, Proteome, Receptors, Immunologic, Salmonella Infections, Animal, Salmonella typhimurium, Transcriptome

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Public Library of Science (PLoS)
Medical Research Council (G0801161)
Isaac Newton Trust (MINUTE 1307(U))
Medical Research Council (G1100102)
Medical Research Council (G0801161/1)
Medical Research Council (G1100102/1)
This work was supported by a Medical Research Council (MRC) grant G0801161awarded to A.J.G., P.M. and D.J.M. OO was supported by a Newton Trust grant awarded to AJG. MC was supported by an Engineering and Physical Sciences Research Council (EPSRC) doctoral training studentship.