Repository logo
 

Infection of Arabidopsis by cucumber mosaic virus triggers jasmonate-dependent resistance to aphids that relies partly on the pattern-triggered immunity factor BAK1.

Accepted version
Peer-reviewed

No Thumbnail Available

Type

Article

Change log

Authors

Tungadi, Trisna 
Watt, Lewis G 
Groen, Simon C 
Du, Zhiyou 

Abstract

Many aphid-vectored viruses are transmitted nonpersistently via transient attachment of virus particles to aphid mouthparts and are most effectively acquired or transmitted during brief stylet punctures of epidermal cells. In Arabidopsis thaliana, the aphid-transmitted virus cucumber mosaic virus (CMV) induces feeding deterrence against the polyphagous aphid Myzus persicae. This form of resistance inhibits prolonged phloem feeding but promotes virus acquisition by aphids because it encourages probing of plant epidermal cells. When aphids are confined on CMV-infected plants, feeding deterrence reduces their growth and reproduction. We found that CMV-induced inhibition of growth as well as CMV-induced inhibition of reproduction of M. persicae are dependent upon jasmonate-mediated signalling. BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) is a co-receptor enabling detection of microbe-associated molecular patterns and induction of pattern-triggered immunity (PTI). In plants carrying the mutant bak1-5 allele, CMV induced inhibition of M. persicae reproduction but not inhibition of aphid growth. We conclude that in wildtype plants CMV induces two mechanisms that diminish performance of M. persicae: a jasmonate-dependent and PTI-dependent mechanism that inhibits aphid growth, and a jasmonate-dependent, PTI-independent mechanism that inhibits reproduction. The growth of two crucifer specialist aphids, Lipaphis erysimi and Brevicoryne brassicae, was not affected when confined on CMV-infected A. thaliana. However, B. brassicae reproduction was inhibited on CMV-infected plants. This suggests that in A. thaliana CMV-induced resistance to aphids, which is thought to incentivize virus vectoring, has greater effects on polyphagous than on crucifer specialist aphids.

Description

Keywords

CMV 2b protein, PAMP-triggered immunity, epidemiology, ethylene, jasmonate, salicylate, Animals, Aphids, Arabidopsis, Arabidopsis Proteins, Cucumovirus, Cyclopentanes, Oxylipins, Plant Diseases, Protein Serine-Threonine Kinases

Journal Title

Mol Plant Pathol

Conference Name

Journal ISSN

1464-6722
1364-3703

Volume Title

22

Publisher

Wiley

Rights

All rights reserved
Sponsorship
Biotechnology and Biological Sciences Research Council (1646532)
Biotechnology and Biological Sciences Research Council (BB/J011762/1)
Leverhulme Trust (F/09 741/F)
Leverhulme Trust (RPF-2012-667)
Isaac Newton Trust (1207(1))
Biotechnology and Biological Sciences Research Council (BB/P023223/1)
European Commission (236443)
Biotechnology and Biological Sciences Research Council (BB/M011194/1)
Work was funded by grants from the UK Biotechnological and Biological Sciences Research Council (SCPRID grant number BB/J011762/1, and GCRF grant number BB/P023223/1: http://www.bbsrc.ac.uk/) and the Leverhulme Trust (F/09741/F, RPG-2012-667: https://www.leverhulme.ac.uk/). SCG had a BBSRC studentship with additional support from the Cambridge European Trust, University of Cambridge Department of Plant Sciences Frank Smart Studentship, and the Cambridge Philosophical Society. LGW was supported by a studentship from the BBSRC-Cambridge University Doctoral Training Program (BB/M011194/1). ZD was a EU Marie Curie International Incoming Fellow (PIIFGA-2009-236443).