Impact of dexamethasone on SARS-CoV-2 concentration kinetics and antibody response in hospitalized COVID-19 patients: results from a prospective observational study.


Type
Article
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Authors
Mühlemann, Barbara 
Thibeault, Charlotte 
Hillus, David 
Helbig, Elisa T 
Lippert, Lena J 
Abstract

OBJECTIVES: Dexamethasone has become the standard of care for severe coronavirus disease 2019 (COVID-19), but its virological impact is poorly understood. The objectives of this work were to characterize the kinetics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) concentration in the upper respiratory tract (URT) and the antibody response in patients with (D+) and without (D-) dexamethasone treatment. METHODS: Data and biosamples from hospitalized patients with severe COVID-19, enrolled between 4th March and 11th December 2020 in a prospective observational study, were analysed. SARS-CoV-2 virus concentration in serial URT samples was measured using RT-PCR. SARS-CoV-2-specific immunoglobulins A and G (IgA and IgG) were measured in serum samples using S1-ELISA. RESULTS: We compared 101 immunocompetent patients who received dexamethasone (according to the inclusion criteria and dosage determined in the RECOVERY trial) to 93 immunocompetent patients with comparable disease severity from the first months of the pandemic, who had not been treated with dexamethasone or other glucocorticoids. We found no inter-group differences in virus concentration kinetics, duration of presence of viral loads >106 viral copies/mL (D+ median 17 days (IQR 13-24), D- 19 days (IQR 13-29)), or time from symptom onset until seroconversion (IgA: D+ median 11.5 days (IQR 11-12), D- 14 days (IQR 11.5-15.75); IgG: D+ 13 days (IQR 12-14.5), D- 12 days (IQR 11-15)). CONCLUSION: Dexamethasone does not appear to lead to a change in virus clearance or a delay in antibody response in immunocompetent patients hospitalized with severe COVID-19.

Description
Keywords
Antibody, COVID-19 nucleic acid testing, Coronavirus disease 2019 (COVID-19), Dexamethasone, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Viral concentration, Anti-Inflammatory Agents, Antibodies, Viral, COVID-19, Dexamethasone, Hospitalization, Humans, Immunoglobulin A, Immunoglobulin G, Kinetics, Prospective Studies, RNA, Viral, Respiratory System, SARS-CoV-2, Seroconversion, Viral Load, COVID-19 Drug Treatment
Journal Title
Clin Microbiol Infect
Conference Name
Journal ISSN
1198-743X
1469-0691
Volume Title
Publisher
Elsevier BV